背景技术硒纳米颗粒(SeNP)是金属纳米颗粒中的一种,被广泛用于抗微生物,抗氧化剂,抗炎活性,和其他生物医学应用。TridaxProcumbens(TP)茎提取物是一种富含类黄酮的有前途的草本植物,单宁,生物碱,植物甾醇,和羟基肉桂酸酯,在伤口愈合应用中起主要作用。目的利用TP茎提取物合成SeNPs,表征,及其生物医学应用。材料和方法使用TP茎提取物合成SeNPs。通过紫外-可见(UV-vis)光谱分析证实了SeNP的绿色合成。使用傅立叶变换红外光谱(FTIR)和扫描电子显微镜(SEM)对合成的SeNPs进行了表征。利用琼脂孔扩散法来评估使用TP茎提取物的绿色合成的SeNPs的抗菌特性。使用2,2-二苯基-1-吡啶酰肼(DPPH)测定法测试了SeNP的抗氧化作用,三价铁还原抗氧化能力测定(FRAP),和羟自由基清除测定(H2O2)。用牛血清白蛋白测定法和卵清蛋白变性法研究其抗炎作用,并且使用盐水虾致死率(BSL)测定法测试绿色合成的SeNPs的细胞毒性作用。结果使用不同类型的分析技术证实了SeNP的绿色合成。通过紫外可见光谱分析进行表征,在330nm的范围内显示最大峰。SEM分析显示纳米颗粒的形状为六边形。琼脂孔扩散法显示了SeNPs对伤口微生物的抗菌功效,对大肠杆菌的抑制作用为14.6mm(E.大肠杆菌),金黄色葡萄球菌15.8毫米(S.金黄色葡萄球菌),铜绿假单胞菌为15.4毫米(P.铜绿假单胞菌)。TP茎介导的SeNPs显示出潜在的抗氧化作用,抗炎,和细胞毒活性,毒性很小。结论总体来说,TP-茎介导的SeNPs的绿色合成在生物医学应用中具有巨大的潜力。因此,合成的SeNPs对伤口病原体表现出显著的抗菌功效。TP茎介导的SeNPs显示出潜在的抗氧化作用,抗炎,和细胞毒活性,显示低毒性。此外,绿色合成的SeNPs可用于治疗管理。
Background Selenium nanoparticles (SeNPs) are one of the metal nanoparticles that have been widely utilized for their anti-microbial, anti-oxidant, anti-inflammatory activities, and other biomedical applications. Tridax procumbens (TP) stem extract is a promising herb species rich in flavonoids, tannins, alkaloids, phytosterols, and hydroxycinnamates, which play a major role in wound healing applications. Aim The study aims to synthesize SeNPs using TP stem extract, characterizations, and its biomedical applications. Materials and methods SeNPs were synthesized using TP stem extract. The green synthesis of SeNPs was confirmed by ultraviolet-visible (UV-vis) spectra analysis. The synthesized SeNPs were characterized using Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The agar well diffusion method was utilized to evaluate the anti-bacterial properties of the green synthesized SeNPs using TP stem extract. The anti-oxidant effect of SeNPs was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, ferric-reducing anti-oxidant power assay (FRAP), and hydroxyl radical scavenging assay (H₂O₂). The anti-inflammatory effect was investigated using the bovine serum albumin assay and egg albumin denaturation method, and the cytotoxic effect of the green synthesized SeNPs was tested using the brine shrimp lethality (BSL) assay. Results The green synthesis of SeNPs was confirmed using different types of analysis techniques. The characterizations were done by UV-visible spectroscopy analysis, exhibiting a maximum peak at the range of 330 nm. SEM analysis revealed the shape of the nanoparticle to be hexagonal. The agar well diffusion method exhibited the anti-bacterial efficacy of SeNPs against wound microorganisms with a zone of inhibition of 14.6 mm for Escherichia coli (E. coli), 15.8 mm for Staphylococcus aureus (S. aureus), and 15.4 mm for Pseudomonas aeruginosa (P. aeruginosa). The TP stem-mediated SeNPs showed potential effects in anti-oxidant, anti-inflammatory, and cytotoxic activity, which shows very little toxicity. Conclusion Overall, the green synthesis of TP-stem-mediated SeNPs has great potential in biomedical applications. Thus, the synthesized SeNPs exhibit significant anti-bacterial efficacy against wound pathogens. The TP stem-mediated SeNPs showed potential effects in anti-oxidant, anti-inflammatory, and cytotoxic activity, which shows low toxicity. Furthermore, the green-synthesized SeNPs can be utilized in therapeutic management.