PDF(Pubmed)
Abstract：
The liver is the most important metabolic organ in the body. While mouse models and cell lines have further deepened our understanding of liver biology and related diseases, they are flawed in replicating key aspects of human liver tissue, particularly its complex structure and metabolic functions. The organoid model represents a major breakthrough in cell biology that revolutionized biomedical research. Organoids are in vitro three-dimensional (3D) physiological structures that recapitulate the morphological and functional characteristics of tissues in vivo, and have significant advantages over traditional cell culture methods. In this review, we discuss the generation strategies and current advances in the field focusing on their application in regenerative medicine, drug discovery and modeling diseases.
摘要：
肝脏是人体最重要的代谢器官。虽然小鼠模型和细胞系进一步加深了我们对肝脏生物学和相关疾病的理解，它们在复制人类肝脏组织的关键方面存在缺陷，特别是其复杂的结构和代谢功能。类器官模型代表了细胞生物学的重大突破，彻底改变了生物医学研究。类器官是体外三维（3D）生理结构，概括了体内组织的形态和功能特征，与传统细胞培养方法相比具有显著优势。在这次审查中,我们讨论了该领域的生成策略和当前进展，重点是它们在再生医学中的应用，药物发现和建模疾病。
