Abstract：
Although the use of thermoplastic polyurethane (Tpu) nanofiber mats as wound dressings is of great interest due to their mechanical properties, they are hindered by their poor wettability and bioavailability. In this study, we aimed to improve the cellular affinity of Tpu nanofiber mats for skin disorders by incorporating extracted collagen (Col) from tendons and physically mixed with a layer of phytoceramides (Phyto) to produce TpuCol@X-Phyto mats in which the weight % of Phyto relatively to the weight of the solution was X = 0.5, 1.0, or 1.5 wt% via facile electrospinning approach. The collective observations strongly indicate the successful incorporation and retention of Phyto within the TpuCol architecture. An increase in the Phyto concentration decreased the water contact angle from 69.4° ± 3.47° to 57.9° ± 2.89°, demonstrating improvement in the hydrophilicity of Tpu and binary blend TpuCol nanofiber mats. The mechanical property of 1.0 wt% Phyto aligns with practical requirements owing to the presence of two hydroxyl groups and the amide linkage likely contributing to various hydrogen bonds, providing mechanical strength to the channel structure and a degree of rigidity essential for transmitting mechanical stress. The proliferation of human skin fibroblast (HSF) peaked significantly 100 % with TpuCol@X-Phyto mats coated for X =1.0 and 1.5 wt% of Phyto. Electrospun scaffolds with the highest Phyto content have shown the lowest degree of hemolysis, demonstrating the high level of compatibility between them and blood. The TpuCol@1.5Phyto mat also demonstrated higher efficacy in antibacterial and antioxidant activities, achieving a rate of DPPH radical scavenging of 83.3 % for this latter property. The most notable wound closure among all tested formulations was attributed to higher Phyto. Thus, the developed TpuCol@1.5Phyto nanofiber formula exhibited enhanced healing in an in vitro epidermal model.
摘要：
尽管由于其机械性能，使用热塑性聚氨酯（Tpu）纳米纤维垫作为伤口敷料引起了极大的兴趣，它们的润湿性和生物利用度差。在这项研究中,我们旨在通过掺入从肌腱中提取的胶原蛋白（Col）并与一层植物神经酰胺（Phyto）物理混合来生产TpuCol@X-Phyto垫，从而提高Tpu纳米纤维垫对皮肤疾病的细胞亲和力。通过简单的电纺方法，Phyto相对于溶液重量的重量百分比为X=0.5、1或1.5wt％。集体观察有力地表明了Phyto在TpuCol体系结构中的成功结合和保留。Phyto浓度的增加使水接触角从69.4°±3.47°降低到57.9°±2.89°，证明了Tpu和二元共混TpuCol纳米纤维垫的亲水性的改善。由于存在两个羟基和酰胺键可能会导致各种氢键，因此1.0wt％Phyto的机械性能符合实际要求。为通道结构提供机械强度和传递机械应力所必需的刚度。人皮肤成纤维细胞（HSF）的增殖显着达到100％的峰值，其中TpuCol@X-Phyto垫涂有X=1.0和1.5wt％的Phyto。植物蛋白含量最高的静电纺支架显示出最低的溶血程度，证明了它们与血液的高度相容性.TpuCol@1.5Phyto垫在抗菌和抗氧化活性方面也表现出更高的功效，对于后一性质,实现83.3%的DPPH自由基清除率。所有测试制剂中最显著的伤口闭合归因于较高的Phyto。因此，开发的TpuCol@1.5Phyto纳米纤维配方在体外表皮模型中显示出增强的愈合。
