目的:评估体内1)通过舌下胶囊给药时反式白藜芦醇的生物利用度;2)白藜芦醇对获得性釉质膜(AEP)蛋白质组成的影响。
方法:10名志愿者接受含50mg反式白藜芦醇的舌下胶囊。然后在0、30、60和120分钟后收集未刺激的唾液,并在施用胶囊后120分钟后收集AEP。下周,志愿者接受了安慰剂舌下胶囊,再次收集唾液和AEP。使用高效液相色谱(HPLC)分析唾液样品中的游离反式白藜芦醇,和AEP样品进行蛋白质组学分析(nLC-ESI-MS/MS)。
结果:在所评估的所有时间点均在唾液中检测到反式白藜芦醇,峰值在30分钟。两组共鉴定出242种蛋白质。白藜芦醇组96种蛋白质增加,23种蛋白质减少。在上调的蛋白质中,半胱氨酸抑制素的同工型,PRPs,粘蛋白-7,组织蛋白-1,乳铁蛋白和溶菌酶-C增加,蛋白S100,中性粒细胞防御素,白蛋白,PRPs,and,白藜芦醇组Statherin降低。
结论:舌下胶囊可有效提高唾液中反式白藜芦醇的生物利用度。鉴定了几种参与维持全身和口腔健康稳态的重要过程的蛋白质。这些蛋白质由于反式白藜芦醇的存在而表达不同,值得未来研究关注。因为它们有重要的功能,主要与抗菌作用有关。
OBJECTIVE: To evaluate in vivo 1) the bioavailability of trans-resveratrol when administered through sublingual capsules; 2) the effect of resveratrol on the protein composition of the acquired enamel pellicle (AEP).
METHODS: Ten volunteers received a sublingual capsule containing 50 mg of trans-resveratrol. Unstimulated saliva was then collected after 0, 30, 60, and 120 min and AEP was collected after 120 min following administration of the capsule. In the next week, the volunteers received a placebo sublingual capsule, and saliva and AEP were collected again. Saliva samples were analyzed for free trans-resveratrol using high-performance liquid chromatopgraphy (HPLC), and AEP samples were subjected to proteomic analysis (nLC-ESI-MS/MS).
RESULTS: Trans-resveratrol was detected in saliva at all the time points evaluated, with the peak at 30 min. A total of 242 proteins were identified in both groups. Ninety-six proteins were increased and 23 proteins were decreased in the Resveratrol group. Among the up-regulated proteins, isoforms of cystatins, PRPs, Mucin-7, Histatin-1, Lactotrasnferrin and Lysozyme-C were increased and the isoforms of Protein S100, Neutrophil defensins, Albumin, PRPs, and, Statherin were decreased in Resveratrol group.
CONCLUSIONS: The sublingual capsule is effective at increasing the bioavailability of trans-resveratrol in saliva. Several proteins involved in important processes to maintain systemic and oral health homeostasis were identified. These proteins differently expressed due to the presence of trans-resveratrol deserve attention for future studies, since they have important functions, mainly related to antimicrobial action.