Abstract：
Aphids are sap-sucking insects responsible for crop losses and a severe threat to crop production. Proteins in the aphid saliva are integral in establishing an interaction between aphids and plants and are responsible for host plant adaptation. The cotton aphid, Aphis gossypii (Hemiptera: Aphididae) is a major pest of Gossypium hirsutum. Despite extensive studies of the salivary proteins of various aphid species, the components of A. gossypii salivary glands are unknown. In this study, we identified 123,008 transcripts from the salivary gland of A. gossypii. Among those, 2933 proteins have signal peptides with no transmembrane domain known to be secreted from the cell upon feeding. The transcriptome includes proteins with more comprehensive functions such as digestion, detoxification, regulating host defenses, regulation of salivary glands, and a large set of uncharacterized proteins. Comparative analysis of salivary proteins of different aphids and other insects with A. gossypii revealed that 183 and 88 orthologous clusters were common in the Aphididae and non-Aphididae groups, respectively. The structure prediction for highly expressed salivary proteins indicated that most possess an intrinsically disordered region. These results provide valuable reference data for exploring novel functions of salivary proteins in A. gossypii with their host interactions. The identified proteins may help develop a sustainable way to manage aphid pests.
摘要：
蚜虫是造成作物损失和对作物生产的严重威胁的吸液昆虫。蚜虫唾液中的蛋白质在建立蚜虫与植物之间的相互作用中是不可或缺的，并且负责宿主植物的适应。棉花蚜虫,蚜虫（半翅目：蚜科）是陆地棉的主要害虫。尽管对各种蚜虫物种的唾液蛋白进行了广泛的研究，A.gossypii唾液腺的组成部分是未知的。在这项研究中,我们鉴定了来自棉曲霉菌唾液腺的123,008个转录物。其中,2933蛋白具有信号肽,其不具有已知在饲喂时从细胞分泌的跨膜结构域。转录组包括具有更全面功能的蛋白质，如消化，排毒,调节宿主防御，唾液腺的调节，和大量未表征的蛋白质。不同蚜虫和其他昆虫的唾液蛋白与棉铃虫的比较分析显示，在蚜虫和非蚜虫组中共有183和88个直系同源簇，分别。高度表达的唾液蛋白的结构预测表明，大多数具有内在无序的区域。这些结果为探索棉草唾液蛋白与宿主相互作用的新功能提供了有价值的参考数据。鉴定的蛋白质可能有助于开发一种可持续的方法来管理蚜虫害虫。
