PDF(Pubmed)
Abstract：
OBJECTIVE: Haemaphysalis longicornis is an obligate blood-sucking ectoparasite that has gained attention due its role of transmitting medically and veterinary significant pathogens and it is the most common tick species in Republic of Korea. The preferred strategy for controlling ticks is a multi-antigenic vaccination. Testing the efficiency of a combination antigen is a promising method for creating a tick vaccine.
OBJECTIVE: The aim of the current research was to analyze the role of subolesin and enolase in feeding and reproduction of H. longicornis by gene silencing.
METHODS: In this study, we used RNA interference to silence salivary enolase and subolesin in H. longicornis. Unfed female ticks injected with double-stranded RNA targeting subolesin and enolase were attached and fed normally on the rabbit\'s ear. Real-time polymerase chain reaction was used to confirm the extent of knockdown.
RESULTS: Ticks in the subolesin or enolase dsRNA groups showed knockdown rates of 80% and 60% respectively. Ticks in the combination dsRNA (subolesin and enolase) group showed an 80% knockdown. Knockdown of subolesin and enolase resulted in significant depletion in feeding, blood engorgement weight, attachment rate, and egg laying. Silencing of both resulted in a significant (p < 0.05) reduction in tick engorgement, egg laying, egg hatching (15%), and reproduction.
CONCLUSIONS: Our results suggest that subolesin and enolase are an exciting target for future tick control strategies.
OBJECTIVE: The aim of the current research was to analyze the role of subolesin and enolase in feeding and reproduction of H. longicornis by gene silencing.
METHODS: In this study, we used RNA interference to silence salivary enolase and subolesin in H. longicornis. Unfed female ticks injected with double-stranded RNA targeting subolesin and enolase were attached and fed normally on the rabbit\'s ear. Real-time polymerase chain reaction was used to confirm the extent of knockdown.
RESULTS: Ticks in the subolesin or enolase dsRNA groups showed knockdown rates of 80% and 60% respectively. Ticks in the combination dsRNA (subolesin and enolase) group showed an 80% knockdown. Knockdown of subolesin and enolase resulted in significant depletion in feeding, blood engorgement weight, attachment rate, and egg laying. Silencing of both resulted in a significant (p < 0.05) reduction in tick engorgement, egg laying, egg hatching (15%), and reproduction.
CONCLUSIONS: Our results suggest that subolesin and enolase are an exciting target for future tick control strategies.
摘要：
目的：长钩病是一种专性吸血外寄生虫，由于其传播医学和兽医学重要病原体的作用而受到关注，并且是大韩民国最常见的蜱类。控制蜱的优选策略是多抗原疫苗接种。测试组合抗原的效率是产生蜱疫苗的有前途的方法。
目的：本研究的目的是通过基因沉默分析subolesin和烯醇化酶在长尾螺旋藻摄食和繁殖中的作用。
方法：在本研究中，我们使用RNA干扰沉默长螺旋藻的唾液烯醇化酶和亚奥菌素。将注射有靶向subolesin和烯醇化酶的双链RNA的未饲喂的雌性蜱附着在兔的耳朵上，并正常喂养。使用实时聚合酶链反应来确认敲除的程度。
结果：在subolesin或烯醇化酶dsRNA组中的Ticks分别显示80%和60%的敲减率。组合dsRNA(subolesin和烯醇化酶)组中的标记显示80%敲低。敲除subolesin和烯醇化酶导致进食的显着消耗，血液充血的重量，依恋率，产蛋。两者的沉默导致蜱充血显著(p<0.05)减少，产蛋,卵孵化（15%），和繁殖。
结论：我们的研究结果表明，subolesin和烯醇化酶是未来蜱控制策略的一个令人兴奋的目标。
目的：本研究的目的是通过基因沉默分析subolesin和烯醇化酶在长尾螺旋藻摄食和繁殖中的作用。
方法：在本研究中，我们使用RNA干扰沉默长螺旋藻的唾液烯醇化酶和亚奥菌素。将注射有靶向subolesin和烯醇化酶的双链RNA的未饲喂的雌性蜱附着在兔的耳朵上，并正常喂养。使用实时聚合酶链反应来确认敲除的程度。
结果：在subolesin或烯醇化酶dsRNA组中的Ticks分别显示80%和60%的敲减率。组合dsRNA(subolesin和烯醇化酶)组中的标记显示80%敲低。敲除subolesin和烯醇化酶导致进食的显着消耗，血液充血的重量，依恋率，产蛋。两者的沉默导致蜱充血显著(p<0.05)减少，产蛋,卵孵化（15%），和繁殖。
结论：我们的研究结果表明，subolesin和烯醇化酶是未来蜱控制策略的一个令人兴奋的目标。
