Abstract：
The α-Gal syndrome (AGS) is an IgE-mediated tick borne-allergy that results in delayed anaphylaxis to the consumption of mammalian meat and products containing α-Gal. Considering that α-Gal-containing microbiota modulates natural antibody production to this glycan, this study aimed to evaluate the influence on tick salivary compounds on the gut microbiota composition in the zebrafish (Danio rerio) animal model. Sequencing of 16 S rDNA was performed in a total of 75 zebrafish intestine samples, representing different treatment groups: PBS control, Ixodes ricinus tick saliva, tick saliva non-protein fraction (NPF), tick saliva protein fraction (PF), and tick saliva protein fractions 1-5 with NPF (F1-5). The results revealed that treatment with tick saliva and different tick salivary fractions, combined with α-Gal-positive dog food feeding, resulted in specific variations in zebrafish gut microbiota composition at various taxonomic levels and affected commensal microbial alpha and beta diversities. Metagenomics results were corroborated by qPCR, supporting the overrepresentation of phylum Firmicutes in the tick saliva group, phylum Fusobacteriota in group F1, and phylum Cyanobacteria in F2 and F5 compared to the PBS-control. qPCRs results at genus level sustained significant enrichment of Plesiomonas spp. in groups F3 and F5, Rhizobium spp. in NPF and F4, and Cloacibacterium spp. dominance in the PBS control group. This study provides new results on the role of gut microbiota in allergic reactions to tick saliva components using a zebrafish model of AGS. Overall, gut microbiota composition in response to tick saliva biomolecules may be associated with allergic reactions to mammalian meat consumption in AGS.
摘要：
α-Gal综合征(AGS)是IgE介导的蜱传播的变态反应,其导致对哺乳动物肉和含有α-Gal的产品的食用的延迟过敏反应。考虑到含α-Gal的微生物群调节这种聚糖的天然抗体产生，本研究旨在评估tick唾液化合物对斑马鱼（Daniorerio）动物模型肠道菌群组成的影响。在总共75个斑马鱼肠道样品中进行了16SrDNA的测序，代表不同的治疗组：PBS对照，蓖麻Ixodestick唾液，蜱唾液非蛋白质部分（NPF），蜱唾液蛋白组分（PF），并用NPF(F1-5)滴答唾液蛋白级分1-5。结果表明，用蜱唾液和不同的蜱唾液部分处理，结合α-Gal阳性狗食喂养，导致斑马鱼肠道菌群组成在各种分类水平上的特定变化，并影响共生微生物α和β多样性。通过qPCR证实了宏基因组学结果，支持壁虱唾液组中Firmicutes的代表过多，与PBS对照相比，F1组中的梭杆菌门和F2和F5中的蓝藻门。属水平的qPCR结果持续显着富集了假单胞菌属。在F3和F5组中，根瘤菌属。在NPF和F4以及回肠杆菌属中。在PBS对照组中占主导地位。这项研究使用斑马鱼AGS模型，为肠道微生物群在tick唾液成分过敏反应中的作用提供了新的结果。总的来说,响应蜱唾液生物分子的肠道微生物群组成可能与AGS中哺乳动物肉类消费的过敏反应有关。
