PDF(Pubmed)
Abstract：
Mucin 7 (MUC7) is one of the salivary proteins whose role in the innate immune system is widely known, but still, neither its mechanism of action nor the impact of its metal coordination is fully understood. MUC7 and its fragments demonstrate potent antimicrobial activity, serving as a natural defense mechanism for organisms against pathogens. This study delves into the bioinorganic chemistry of MUC7 fragments (L1─EGRERDHELRHRRHHHQSPK; L2─EGRERDHELRHRR; L3─HHHQSPK) and their complexes with Cu(II) and Zn(II) ions. The antimicrobial characteristics of the investigated peptides and their complexes were systematically assessed against bacterial and fungal strains at pH 5.40 and pH 7.40. Our findings highlight the efficacy of these systems against Streptococcus sanguinis, a common oral cavity pathogen. Most interestingly, Zn(II) coordination increased (or triggered) the MUC7 antimicrobial activity, which underscores the pivotal role of metal ion coordination in governing the antimicrobial activity of human salivary MUC7 fragments against S. sanguinis.
摘要：
粘蛋白7（MUC7）是唾液蛋白之一，其在先天免疫系统中的作用是众所周知的，但仍然,它的作用机理和金属配位的影响都不完全清楚。MUC7及其片段显示出有效的抗微生物活性，作为生物体对抗病原体的天然防御机制。本研究探讨了MUC7片段（L1─EGRERDHELRHRRHHQSPK；L2─EGRERDHELRHQSPK；L3─HHQSPK）及其与Cu（II）和Zn（II）离子的络合物的生物无机化学。在pH5.40和pH7.40对细菌和真菌菌株系统地评估了所研究的肽及其复合物的抗微生物特性。我们的发现强调了这些系统对血链球菌的功效，一种常见的口腔病原体。最有趣的是，Zn（II）配位增加（或触发）MUC7抗菌活性，这强调了金属离子配位在控制人唾液MUC7片段抗血红链球菌的抗菌活性中的关键作用。
