苦瓜属属于荨麻科中的苦瓜属,是一种营养价值很高的优质野菜。在这项研究中,高通量技术被用来测序,组装并注释叶绿体基因组。我们还分析了它的结构,并从沙棘上构建系统发育树,以进一步研究叶绿体基因组特征。结果表明,叶绿体基因组大小为153220bp,GC含量为36.4%,属于P.scabra中典型的四分体结构。叶绿体基因组编码130个基因,包括85个蛋白质编码基因,37个tRNA基因,和8个rRNA基因。其中,15个基因含有1个内含子,2个基因含有2个内含子,rps12有反式剪接,分别。在P.scabra中,叶绿体基因组可分为四类,包括43个光合作用,64自我复制,其他7种编码蛋白,4个未知函数在叶绿体基因组中检测到51073个密码子,其中密码子编码亮氨酸(Leu)所占比例最大,密码子优选使用A和U碱基。在沙棘的叶绿体基因组中有72个简单序列重复(SSRs),含有58个单核苷酸,12二核苷酸,1个三核苷酸,和1个四核苷酸。ycf1基因扩增存在于IRb/SSC边界处。系统发育树显示,苦参(OL800583)与苦参(MZ292972)关系最密切,口蹄疫(MK227819)和口蹄疫。lavissimum(MN189961)。一起来看,我们的结果为理解识别提供了有价值的信息,遗传进化,和沙棘菌种的基因组学研究。
Pellionia scabra belongs to the genus Pellionia in the family of Urticaceae, and is a high-quality wild vegetables with high nutritional value. In this study, high-throughput techniques were used to sequence, assemble and annotate the chloroplast genome. We also analyzed its structure, and construct the phylogenetic trees from the P. scabra to further study the chloroplast genome characteristics. The results showed that the chloroplast genome size was 153 220 bp, and the GC content was 36.4%, which belonged to the typical tetrad structure in P. scabra. The chloroplast genome encodes 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes in P. scabra. Among them, 15 genes contained 1 intron, 2 genes contained 2 introns, and rps12 had trans-splicing, respectively. In P. scabra, chloroplast genomes could be divided into four categories, including 43 photosynthesis, 64 self-replication, other 7 coding proteins, and 4 unknown functions. A total of 51 073 codons were detected in the chloroplast genome, among which the codon encoding leucine (Leu) accounted for the largest proportion, and the codon preferred to use A and U bases. There were 72 simple sequence repeats (SSRs) in the chloroplast genome of P. scabra, containing 58 single nucleotides, 12 dinucleotides, 1 trinucleotide, and 1 tetranucleotide. The ycf1 gene expansion was present at the IRb/SSC boundary. The phylogenetic trees showed that P. scabra (OL800583) was most closely related to Elatostema stewardii (MZ292972), Elatostema dissectum (MK227819) and Elatostema laevissimum var. laevissimum (MN189961). Taken together, our results provide worthwhile information for understanding the identification, genetic evolution, and genomics research of P. scabra species.