Abstract：
The effective utilization of traditional Chinese medicine (TCM) has been challenged by the difficulty to accurately distinguish between similar plant varieties. The stability and conservation of the chloroplast genome can aid in resolving genotypes. Previous studies using nuclear sequences and molecular markers have not effectively differentiated the species from related taxa, such as Machilus leptophylla, Hanceola exserta, Rubus bambusarum, and Rubus henryi. This study aimed to characterize the chloroplast genomes of these four plant species, and analyze their simple sequence repeats (SSRs) and phylogenetic positions. The results demonstrated the four chloroplast genomes consisted of 152.624 kb, 153.296 kb, 156.309 kb, and 158.953 kb in length, involving 124, 130, 129, and 131 genes, respectively. They also contained four specific regions with mononucleotide being the class with the most members. Moreover, these repeating types of SSR were various in individual class. Phylogenetic analysis showed that M. leptophylla was clustered with M. yunnanensis, and H. exserta was confirmed as belonging to the family Ocimeae. Additionally, R. bambusarum and R. henryi were grouped together but differed in their SSR features, indicating that they were not the same species. This research provides evidence for resolving species and contributes new genetic information for further studies.
摘要：
中药（TCM）的有效利用受到难以准确区分相似植物品种的挑战。叶绿体基因组的稳定性和保守性可以帮助解析基因型。以前使用核序列和分子标记的研究尚未有效区分该物种与相关分类群，比如Machilusleptophylla，HanceolaExserta,Rubusbambusarum,还有Rubushenryi.本研究旨在表征这四种植物的叶绿体基因组，并分析其简单序列重复(SSRs)和系统发育位置。结果表明，四个叶绿体基因组由152.624kb组成，153.296kb,156.309kb,和158.953kb的长度，涉及124、130、129和131个基因，分别。它们还包含四个特定区域，单核苷酸是成员最多的类别。此外，这些重复类型的SSR在各个类别中是不同的。系统发育分析表明，轻子与云南M.成簇，和H.exserta被确认属于Ocimeae家族。此外,R.bambusarum和R.henryi被分组在一起，但在SSR特征上有所不同，表明它们不是同一物种。这项研究为解析物种提供了证据，并为进一步研究提供了新的遗传信息。
