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Abstract：
Chinese black truffle (Tuber indicum) is a hypogenous fungus of great value due to its distinctive aroma. In this study, both transcriptome and physicochemical analyses were performed to investigate the changes of nutrients and gene expression in truffle fruiting bodies during cold storage. The results of physicochemical analysis revealed the active metabolism of fruiting bodies in cold storage, showing the decreased contents of protein and soluble sugar, the variations in both polyphenol oxidase activity and total phenol content, and the detrimental effect of reactive oxygen species production caused by heavy metals (cadmium and lead) in truffles. Transcriptome analysis identified a total of 139,489 unigenes. Down-regulated expression of genes encoding the catalase-like domain-containing protein (katE), glutaredoxin protein (GRX), a copper/zinc superoxide dismutase (Sod_Cu), and aspartate aminotransferase (AAT) affected the degradation metabolism of intracellular oxides. Ribulose-5-phosphate-3-epimerase (RPE) was a key enzyme in response to oxidative stress in truffle cells through the pentose phosphate pathway (PPP). A total of 51,612 simple sequence repeats were identified, providing valuable resources for further genetic diversity analysis, molecular breeding, and genetic map-ping in T. indicum. Transcription factors GAL4 and SUF4-like protein were involved in glucose metabolism and histone methylation processes, respectively. Our study provided a fundamental characterization of the physicochemical and molecular variations in T. indicum during the cold storage at 4°C, providing strong experimental evidence to support the improvement of storage quality of T. indicum.
摘要：
中国黑松露（Tuberindicum）是一种具有独特香气的低生真菌，具有很高的价值。在这项研究中,进行了转录组和理化分析，以研究冷藏过程中块菌子实体中养分和基因表达的变化。理化分析结果表明，子实体在冷藏过程中的活跃代谢，蛋白质和可溶性糖含量下降，多酚氧化酶活性和总酚含量的变化，以及松露中重金属（镉和铅）对活性氧产生的有害影响。转录组分析鉴定了总共139,489个单基因。编码含过氧化氢酶样结构域蛋白（katE）的基因表达下调，谷氧还蛋白（GRX），铜/锌超氧化物歧化酶（Sod_Cu），天冬氨酸转氨酶(AAT)影响细胞内氧化物的降解代谢。核酮糖-5-磷酸-3-差向异构酶(RPE)是响应松露细胞通过戊糖磷酸途径(PPP)的氧化应激的关键酶。总共鉴定了51,612个简单序列重复，为进一步的遗传多样性分析提供宝贵的资源，分子育种,和T.indicum的遗传图谱。转录因子GAL4和SUF4样蛋白参与糖代谢和组蛋白甲基化过程,分别。我们的研究提供了在4°C冷藏期间T.in的物理化学和分子变化的基本表征，提供了有力的实验证据来支持T.in的贮藏质量的提高。
