simple sequence repeat

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  • 文章类型: Journal Article
    保护濒危软体动物的转录组研究是管理自然环境中这些物种面临的威胁和不确定性的一种积极方法。由于栖息地的破坏,这些物种的种群正在减少,非法野生动物贸易,和全球气候变化。这些活动危及物种在野外景观中的自由移动,失去了繁殖地,以及显示对动物福利至关重要的生理属性的限制。腹足类动物面临着最负面的生态影响,并根据其种群动态在过去几年中加入了韩国的保护性物种联盟。此外,由于这些物种的遗传资源受到限制,通过知情规划进行保护是不可能的。这篇评论提供了对韩国受威胁物种倡议下的活动的见解,并特别提到了濒危软体动物的转录组集合。腹足类动物,如中国艾兰,白伊吉斯塔,AegistaQuelpartensis,Incilariafruhstorferi,Koreanohadrakurodana,Satsumamyomphala,和克莱顿逆行已经代表。此外,还讨论了双壳类Cristariaplicata和CaenogastropodaCharonialampassauliae的转录组摘要。测序,从头大会,并注释确定了该物种的转录本或同源物,基于对生化和分子途径的理解,被归因于预测基因功能。从转录组中挖掘简单的序列重复序列已成功地辅助了遗传多态性研究。已通过同源性和类比讨论了韩国濒危软体动物的转录组方案与其他濒危软体动物的基因组资源的比较,以指示未来的研究。
    Transcriptome studies for conservation of endangered mollusks is a proactive approach towards managing threats and uncertainties facing these species in natural environments. The population of these species is declining due to habitat destruction, illicit wildlife trade, and global climate change. These activities risk the free movement of species across the wild landscape, loss of breeding grounds, and restrictions in displaying the physiological attributes so crucial for faunal welfare. Gastropods face the most negative ecological effects and have been enlisted under Korea\'s protective species consortium based on their population dynamics in the last few years. Moreover, with the genetic resources restricted for such species, conservation by informed planning is not possible. This review provides insights into the activities under the threatened species initiative of Korea with special reference to the transcriptome assemblies of endangered mollusks. The gastropods such as Ellobium chinense, Aegista chejuensis, Aegista quelpartensis, Incilaria fruhstorferi, Koreanohadra kurodana, Satsuma myomphala, and Clithon retropictus have been represented. Moreover, the transcriptome summary of bivalve Cristaria plicata and Caenogastropoda Charonia lampas sauliae is also discussed. Sequencing, de novo assembly, and annotation identified transcripts or homologs for the species and, based on an understanding of the biochemical and molecular pathways, were ascribed to predictive gene function. Mining for simple sequence repeats from the transcriptome have successfully assisted genetic polymorphism studies. A comparison of the transcriptome scheme of Korean endangered mollusks with the genomic resources of other endangered mollusks have been discussed with homologies and analogies for dictating future research.
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  • 文章类型: Journal Article
    栗子(栗子。,菊科)是经济和生态上有价值的物种。板栗生产的主要目标因物种和国家而异,并取决于果园的生态特征,农艺管理,和栗树的建筑。这里,我们回顾了最近对栗树的研究,包括真菌病(寄生虫和肉桂疫霉)和害虫(DryocosuskuriphilphilusYasumatsu)的影响,育种的分子标记,生态效应,内生真菌,和提取物对人体健康有益。我们还回顾了板栗在食品科学领域的研究,技术改进,用于板栗生产的土壤和肥料,和栗子的采后生物学。我们注意到各地区影响板栗生产的因素存在差异,包括中国,美洲,和欧洲,特别是在疾病和害虫的病原体中。例如,在亚洲,板栗对寄生虫的抗性有很大的不同,欧洲,和美国国家。我们的评论为中国栗树的病虫害综合管理提供了新的见解。我们希望这次审查将促进区域之间的合作,并有助于澄清各国在育种努力方向上的差异。
    Chestnut (Castanea spp., Fagaceae family) is an economically and ecologically valuable species. The main goals of chestnut production vary among species and countries and depend on the ecological characteristics of orchards, agronomic management, and the architecture of chestnut trees. Here, we review recent research on chestnut trees, including the effects of fungal diseases (Cryphonectria parasitica and Phytophthora cinnamomi) and insect pests (Dryocosmus kuriphilus Yasumatsu), molecular markers for breeding, ecological effects, endophytic fungi, and extracts with human health benefits. We also review research on chestnut in the food science field, technological improvements, the soil and fertilizer used for chestnut production, and the postharvest biology of chestnut. We noted differences in the factors affecting chestnut production among regions, including China, the Americas, and Europe, especially in the causal agents of disease and pests. For example, there is a major difference in the resistance of chestnut to C. parasitica in Asian, European, and American countries. Our review provides new insights into the integrated disease and pest management of chestnut trees in China. We hope that this review will foster collaboration among regions and help to clarify differences in the direction of breeding efforts among countries.
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  • 文章类型: Journal Article
    DNA is present in most of the cells in our body, which is unique in each and every individual, and we leave a trail of it everywhere we go. This has become an advantage for forensic investigators who use DNA to draw conclusion in identification of victim and accused in crime scenes. This review described the use of genetic markers in forensic investigation and their limitations.
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  • 文章类型: Journal Article
    Sequencing of rice genome has facilitated the understanding of rice evolution and has been utilized extensively for mining of DNA markers to facilitate marker-assisted breeding. Simple sequence repeat (SSR) markers that are tandemly repeated nucleotide sequence motifs flanked by unique sequences are presently the maker of choice in rice improvement due to their abundance, co-dominant inheritance, high levels of allelic diversity, and simple reproducible assay. The current level of genome coverage by SSR markers in rice is sufficient to employ them for genotype identification and marker-assisted selection in breeding for mapping of genes and quantitative trait loci analysis. This review provides comprehensive information on the mapping and applications of SSR markers in investigation of rice cultivars to study their genetic divergence and marker-assisted selection of important agronomic traits.
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