条锈病(又称黄锈病),由病原体Pucciniastriiformisf.sp.小麦(Pst),是世界范围内小麦(TriticumaestivumL.)常见且严重的真菌病。为了识别有效的抗条锈性位点,基于多样性阵列技术和简单序列重复标记,使用来自中国黄河和淮河流域的152个小麦地方品种进行了全基因组关联研究。在五种环境中,对田间条件下成年植物阶段对条锈病的抗性程度进行了表型评估。总的来说,19种加入显示稳定,在多环境田间评估中,在成年植物阶段暴露于Pst的混合种族时,对条锈病发育的抵抗力很高。标记性状关联分析表明,51个基因座与成株植物对条锈病的抗性显着相关。这些基因座包括40个成年植物抗性的数量性状基因座(QTL)区域。20个已确定的抗性QTL与先前报道的黄锈病抗性基因或QTL区域紧密相关,它们分布在1B号染色体上,1D,2A,2B,3A,3B,4A,4B,5B,6B,7A,7B,7D。在染色体1B上检测到6个多性状QTL,1D,2B,3A,3B,7D。二十个QTL定位在1D染色体上,2A,2D,4B,5B,6A,6B,6D,7A,7B,7D,与先前确定的抗黄锈病基因相距甚远。因此,这些QTL是潜在的抗条纹锈病的新基因座。在20个潜在的新QTL中,五(QDS。sicau-2A,QIT。sicau-4B,QDS.sicau-4B.2,QDS.sicau-6A.3和季度。sicau-7D)在至少两种环境中与成年植物阶段的田间响应有关,并可能对条锈性有很大影响。成株植物抗条锈病的新型有效QTL将提高对控制条锈病传播的遗传机制的认识,并将有助于基于分子标记辅助选择的小麦抗条锈病育种。
Stripe rust (also known as yellow rust), caused by the pathogen Puccinia striiformis f. sp. tritici (Pst), is a common and serious fungal disease of wheat (Triticum aestivum L.) worldwide. To identify effective stripe rust resistance loci, a genome-wide association
study was performed using 152 wheat landraces from the Yellow and Huai River Valleys in China based on Diversity Arrays Technology and simple sequence repeat markers. Phenotypic evaluation of the degree of resistance to stripe rust at the adult-plant stage under field conditions was carried out in five environments. In total, 19 accessions displayed stable, high degrees of resistance to stripe rust development when exposed to mixed races of Pst at the adult-plant stage in multi-environment field assessments. A marker-trait association analysis indicated that 51 loci were significantly associated with adult-plant resistance to stripe rust. These loci included 40 quantitative trait loci (QTL) regions for adult-plant resistance. Twenty identified resistance QTL were linked closely to previously reported yellow rust resistance genes or QTL regions, which were distributed across chromosomes 1B, 1D, 2A, 2B, 3A, 3B, 4A, 4B, 5B, 6B, 7A, 7B, and 7D. Six multi-trait QTL were detected on chromosomes 1B, 1D, 2B, 3A, 3B, and 7D. Twenty QTL were mapped to chromosomes 1D, 2A, 2D, 4B, 5B, 6A, 6B, 6D, 7A, 7B, and 7D, distant from previously identified yellow rust resistance genes. Consequently, these QTL are potentially novel loci for stripe rust resistance. Among the 20 potentially novel QTL, five (QDS.sicau-2A, QIT.sicau-4B, QDS.sicau-4B.2, QDS.sicau-6A.3, and QYr.sicau-7D) were associated with field responses at the adult-plant stage in at least two environments, and may have large effects on stripe rust resistance. The novel effective QTL for adult-plant resistance to stripe rust will improve understanding of the genetic mechanisms that control the spread of stripe rust, and will aid in the molecular marker-assisted selection-based breeding of wheat for stripe rust resistance.