花序的形态结构影响种子的生产。苜蓿(紫花苜蓿)花序伸长的调节机制尚不清楚。因此,在这项研究中,我们对转录组进行了比较分析,蛋白质组,和两个极端材料在三个发育阶段的代谢组,以探索紫花苜蓿花序伸长的机制。我们观察了长花序和短花序的发育过程,发现长花序的苜蓿的伸长能力强于短花序的苜蓿。此外,转录组和蛋白质组的综合分析表明,类苯丙素生物合成途径与花序的结构形成密切相关。此外,我们基于苯丙素生物合成中涉及的差异表达基因和蛋白质(DEGs和DEPs),确定了与木质素生物合成相关的关键基因和蛋白质。此外,靶向激素代谢组分析显示IAA,GA,CK在苜蓿花序的花序梗伸长中起重要作用。基于组学分析,我们检测了与植物激素生物合成和信号转导相关的关键基因和蛋白。从WGCNA和WPCNA的结果来看,我们进一步筛选了与木质素生物合成相关的28个候选基因和6个关键蛋白,植物激素生物合成,和信号通路。此外,使用相关性分析发现了19个关键转录因子,它们可能在调节候选基因中起作用。本研究揭示了苜蓿花序伸长的分子机制,为提高苜蓿种子产量奠定了理论基础。
The morphological architecture of
inflorescence influences seed production. The regulatory mechanisms underlying alfalfa (Medicago sativa)
inflorescence elongation remain unclear. Therefore, in this study, we conducted a comparative analysis of the transcriptome, proteome, and metabolome of two extreme materials at three developmental stages to explore the mechanisms underlying
inflorescence elongation in alfalfa. We observed the developmental processes of long and short inflorescences and found that the elongation capacity of alfalfa with long
inflorescence was stronger than that of alfalfa with short inflorescences. Furthermore, integrative analysis of the transcriptome and proteome indicated that the phenylpropanoid biosynthesis pathway was closely correlated with the structural formation of the
inflorescence. Additionally, we identified key genes and proteins associated with lignin biosynthesis based on the differential expressed genes and proteins (DEGs and DEPs) involved in phenylpropanoid biosynthesis. Moreover, targeted hormone metabolome analysis revealed that IAA, GA, and CK play an important role in the peduncle elongation of alfalfa inflorescences. Based on omics analysis, we detected key genes and proteins related to plant hormone biosynthesis and signal transduction. From the WGCNA and WPCNA results, we furthermore screened 28 candidate genes and six key proteins that were correlated with lignin biosynthesis, plant hormone biosynthesis, and signaling pathways. In addition, 19 crucial transcription factors were discovered using correlation analysis that might play a role in regulating candidate genes. This study provides insight into the molecular mechanism of inflorescence elongation in alfalfa and establishes a theoretical foundation for improving alfalfa seed production.