PDF(Pubmed)
Abstract：
Photoperiod insensitivity has been selected by breeders to help adapt crops to diverse environments and farming practices. In wheat, insensitive alleles of Photoperiod-1 (Ppd-1) relieve the requirement of long daylengths to flower by promoting expression of floral promoting genes early in the season; however, these alleles also limit yield by reducing the number and fertility of grain-producing florets through processes that are poorly understood. Here, we performed transcriptome analysis of the developing inflorescence using near-isogenic lines that contain either photoperiod-insensitive or null alleles of Ppd-1, during stages when spikelet number is determined and floret development initiates. We report that Ppd-1 influences the stage-specific expression of genes with roles in auxin signaling, meristem identity, and protein turnover, and analysis of differentially expressed transcripts identified bZIP and ALOG transcription factors, namely PDB1 and ALOG1, which regulate flowering time and spikelet architecture. These findings enhance our understanding of genes that regulate inflorescence development and introduce new targets for improving yield potential.
摘要：
育种者选择了光周期不敏感性，以帮助作物适应不同的环境和耕作方式。在小麦中，光周期-1（Ppd-1）的不敏感等位基因通过在季节早期促进花卉促进基因的表达来减轻对长日长花的要求；但是，这些等位基因也限制了产量通过减少谷物生产小花的数量和生育力，通过很少了解的过程。这里,在确定小穗数和小花发育开始的阶段，我们使用包含光周期不敏感或Ppd-1无效等位基因的近等基因系对发育中的花序进行了转录组分析。我们报告说，Ppd-1影响在生长素信号传导中起作用的基因的阶段特异性表达，分生组织身份，和蛋白质周转，和差异表达转录分析鉴定bZIP和ALOG转录因子，即PDB1和ALOG1，它们调节开花时间和小穗结构。这些发现增强了我们对调节花序发育的基因的理解，并引入了提高产量潜力的新靶标。
