Abstract：
Phytocannabinoids occurring in Cannabis Sativa L. are unique secondary metabolites possessing interesting pharmacological activities. In this study, the dynamics of thermally induced (60 and 120 °C) phytocannabinoid reactions in four cannabis varieties were investigated. Using UHPLC-HRMS/MS, 40 phytocannabinoids were involved in target analysis, and an additional 281 compounds with cannabinoid-like structures and 258 non-cannabinoid bioactive compounds were subjected to suspect screening. As expected, the key reaction was the decarboxylation of acidic phytocannabinoids. Nevertheless, the rate constants differed among cannabis varieties, documenting the matrix-dependence of this process. Besides neutral counterparts of acidic species, ́neẃ bioactive compounds such as hydroxyquinones were found in heated samples. In addition, changes in other bioactive compounds with both cannabinoid-like and non-cannabinoid structures were documented during cannabis heating at 120 °C. The data document the complexity of heat-induced processes and provide a further understanding of changes in bioactivities occurring under such conditions.
摘要：
大麻中存在的植物大麻素是具有有趣药理活性的独特次级代谢产物。在这项研究中,研究了四个大麻品种中热诱导（60和120°C）植物大麻素反应的动力学。采用UHPLC-HRMS/MS,40种植物大麻素参与目标分析，另外281种具有大麻素样结构的化合物和258种非大麻素生物活性化合物进行了可疑筛选。不出所料,关键反应是酸性植物大麻素的脱羧反应。然而，速率常数在大麻品种之间有所不同，记录此过程的矩阵依赖性。除了酸性物种的中性对应物，在加热的样品中发现了生物活性化合物，如羟醌。此外,在大麻在120°C加热期间，记录了具有大麻素样和非大麻素结构的其他生物活性化合物的变化。数据记录了热诱导过程的复杂性，并进一步了解了在这种条件下发生的生物活性变化。
