富含CBD的大麻菌株的分泌腔内容物和风干花序提取物的研究,大麻cv.\'樱桃酒\',进行比较两组之间的酸性大麻素的脱羧率。通过玻璃微毛细血管从头状茎状腺毛中获得的分泌腔内容物,通过高压液相色谱法分析在室温下在黑暗中风干15天的花序样品。与分泌腔样品相比,在空气干燥的花序样品中,酸性大麻素与总大麻素的比率在0.5%至2.4%的范围内如下。在分泌腔内容物中,酸性大麻素占总大麻素的百分比被测量为86.4%大麻二酚酸(CBDA),6.5%四氢大麻酚酸(THCA),4.3%大麻色甲酸(CBCA),1.4%大麻酚酸(CBGA),和0.6%的大麻二酚酸(CBDVA),分别。在风干的花序中,然而,用84%的CBDA检测到酸性大麻素,4.8%THCA,3.3%CBCA,0.8%CBGA,和0.3%Δ9-四氢甘蓝酸(Δ9-THCVA),分别。在分泌腔内容物中,大麻二酚(CBD)与大麻二酚(CBDA)的比例接近1:99(w/w),然而,在风干的花序中大约为1:20(w/w)。此外,仅在风干的花序样品中检测到Δ9-四氢大麻酚(Δ9-THCV)和Δ9-四氢大麻酚(Δ9-THCVA),Δ9-THCV与Δ9-THCVA的比率为约1:20(w/w)。此外,仅在分泌腔内容物中观察到大麻二酚酸(CBDVA)。
Studies with secretory cavity contents and air-dried
inflorescence extracts of the CBD-rich hemp strain, Cannabis sativa cv. \'Cherry Wine\', were conducted to compare the decarboxylation rates of acidic cannabinoids between two groups. The secretory cavity contents acquired from the capitate-stalked glandular trichomes by glass microcapillaries, and
inflorescence samples air-dried for 15 days of storage in darkness at room temperature were analysed by high-pressure liquid chromatography. The ratio of acidic cannabinoids to the total cannabinoids was ranging from 0.5% to 2.4% lower in the air-dried
inflorescence samples compared to the secretory cavity samples as follows. In the secretory cavity content, the percentage of acidic cannabinoids to the total cannabinoids was measured as 86.4% cannabidiolic acid (CBDA), 6.5% tetrahydrocannabinolic acid (THCA), 4.3% cannabichromenic acid (CBCA), 1.4% cannabigerolic acid (CBGA), and 0.6% cannabidivarinic acid (CBDVA), respectively. In the air-dried
inflorescence, however, the acidic cannabinoids were detected with 84% CBDA, 4.8% THCA, 3.3% CBCA, 0.8% CBGA, and 0.3% Δ9-tetrahydrocannabivarinic acid (Δ9-THCVA), respectively. The ratio of cannabidiol (CBD) to cannabidiolic acid (CBDA) was close to 1:99 (w/w) in secretory cavity contents, however, it was roughly 1:20 (w/w) in the air-dried
inflorescence. In addition, Δ9-tetrahydrocannabivarin (Δ9-THCV) and Δ9-tetrahydrocannabivarinic acid (Δ9-THCVA) were only detected in the air-dried inflorescence sample, and the ratio of Δ9-THCV to Δ9-THCVA was about 1:20 (w/w). Besides, cannabidivarinic acid (CBDVA) was only observed in the secretory cavity content.