PDF(Pubmed)
Abstract：
Chronic oral inflammation and biofilm-mediated infections drive diseases such as dental caries and periodontitis. This study investigated the anti-inflammatory and antibacterial potential of an ethanol extract from Astilbe chinensis inflorescence (GA-13-6) as a prominent candidate for natural complex substances (NCS) with therapeutic potential. In LPS-stimulated RAW 264.7 macrophages, GA-13-6 significantly suppressed proinflammatory mediators, including interleukin-6 (IL-6), tumor necrosis factor (TNF), and nitric oxide (NO), surpassing purified astilbin, a known bioactive compound found in A. chinensis. Furthermore, GA-13-6 downregulated the expression of cyclooxygenase-2 (COX2) and inducible nitric oxide synthase (iNOS), indicating an inhibitory effect on the inflammatory cascade. Remarkably, GA-13-6 exhibited selective antibacterial activity against Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis, key players in dental caries and periodontitis, respectively. These findings suggest that complex GA-13-6 holds the potential for the treatment or prevention of periodontal and dental diseases, as well as various other inflammation-related conditions, while averting the induction of antibiotic resistance.
摘要：
慢性口腔炎症和生物膜介导的感染导致龋齿和牙周炎等疾病。这项研究调查了来自Astilbechinensis花序（GA-13-6）的乙醇提取物作为具有治疗潜力的天然复合物质（NCS）的突出候选物的抗炎和抗菌潜力。在LPS刺激的RAW264.7巨噬细胞中，GA-13-6显著抑制促炎介质，包括白细胞介素-6（IL-6），肿瘤坏死因子(TNF),和一氧化氮（NO），超越纯化的astilbin,在A.chinensis中发现的一种已知的生物活性化合物。此外，GA-13-6下调环氧合酶-2（COX2）和诱导型一氧化氮合酶（iNOS）的表达，表明对炎症级联反应的抑制作用。值得注意的是,GA-13-6对变形链球菌具有选择性抗菌活性，血链球菌,牙龈卟啉单胞菌，龋齿和牙周炎的关键人物，分别。这些发现表明，复杂的GA-13-6具有治疗或预防牙周和牙齿疾病的潜力，以及其他各种与炎症相关的疾病，同时避免诱导抗生素耐药性。
