Abstract：
This is the first attempt to report the co-occurrence of somatic embryos, shoots, and inflorescences and their sequential development from stem cell niches of an individual callus mass through morpho-histological study of any angiosperm. In the presence of a proper auxin/cytokinin combination, precambial stem cells from the middle layer of a compact callus, which was derived from the thin cell layer of the inflorescence rachis of Limonium, expressed the highest level of totipotency and pluripotency and simultaneously developed somatic embryos, shoots, and inflorescences. This study also proposed the concept of programmed cell death during bipolar somatic embryo and unipolar shoot bud pattern formation. The unique feature of this research was the stepwise histological description of in vitro racemose inflorescence development. Remarkably, during the initiation of inflorescence development, either a unipolar structure with open vascular elements or an independent bipolar structure with closed vascular elements were observed. The protocol predicted the production of 6.6 ± 0.24 and 7.4 ± 0.24 somatic embryos and shoots, respectively, from 400 mg of callus, which again multiplied, rooted, and acclimatised. The plants\' ploidy level and genetic fidelity were assessed randomly before acclimatisation by flow cytometry and inter simple sequence repeats (ISSR) marker analysis. Finally, the survivability and flower quality of the regenerated plants were evaluated in the field.
摘要：
这是首次尝试报道体细胞胚的共现，射击，和花序及其通过对任何被子植物的形态组织学研究从单个愈伤组织的干细胞壁龛中依次发育。在存在适当的生长素/细胞分裂素组合的情况下，来自致密愈伤组织中间层的胫骨前干细胞，它来自补血草的花序轴的薄细胞层，表达了最高水平的全能性和多能性，同时发育的体细胞胚，射击，和花序。本研究还提出了双极体细胞胚胎和单极芽芽模式形成过程中程序性细胞死亡的概念。这项研究的独特之处在于对体外总状花序发育的逐步组织学描述。值得注意的是,在花序发育的开始，观察到具有开放血管元素的单极结构或具有闭合血管元素的独立双极结构。该方案预测了6.6±0.24和7.4±0.24体细胞胚和芽的产生，分别,从400毫克的愈伤组织，再次成倍增加，根植,并适应。在适应之前，通过流式细胞术和简单序列重复（ISSR）标记分析随机评估植物的倍性水平和遗传保真度。最后,在田间评估了再生植物的生存能力和花质量。
