目的:比较孕龄小于20周的自然流产妇女与相同孕龄的继续妊娠妇女的阴道微生物组。
方法:横断面比较研究设计。
方法:这项研究是在北卡纳塔克邦的一家三级医院进行的,印度。在这个农村地区,许多妇女缺乏产前保健和妇女健康信息的意识。鉴于自然流产率上升,研究阴道微生物组对妊娠结局的影响对于提高当地意识至关重要.
方法:200名妇女;100名在妊娠20周内经历了自然流产,和100个保持相同胎龄的怀孕。
方法:使用阴道拭子检测参与者的阴道微生物群。从阴道样品中提取DNA;使用16S核糖体RNA测序方法鉴定存在的生物。
结果:在阴道培养物中发现病原体检出率为13.15%(n=200)。自然流产妇女和继续妊娠妇女的细菌检出率分别为19.9%和7.9%,分别(p<0.05)。与持续怀孕的女性相比,经历自然流产的妇女有五种常见的物种:大肠杆菌(48%),粪肠球菌(28%),屎肠球菌(12%),荧光假单胞菌(7%),和Cedrina假单胞菌(5%);持续怀孕的人中最普遍的物种是乳酸杆菌(58%),卷曲乳杆菌(32%),和大肠杆菌(10%;p<0.05)。
结论:自然流产妇女的阴道微生物组更加多样化,细菌分布不规则。鉴定的细菌可能会影响女性的妊娠结局,并可能与自然流产有关。护士必须为处于生育期的妇女提供有关产前筛查的教育,性传播感染,和会阴护理.
OBJECTIVE: To compare the vaginal microbiome in women who have undergone spontaneous abortion with a gestational age of less than 20 weeks to that of women who have a continued pregnancy of the same gestational age.
METHODS: Cross-sectional comparative research design.
METHODS: The study was conducted in a tertiary care hospital of North Karnataka, India. In this rural area, many women lack awareness of prenatal care and women\'s health information. Given rising rates of spontaneous abortion, studying the impact of the vaginal microbiome on pregnancy outcomes is crucial for raising local awareness.
METHODS: Two hundred women; 100 experienced spontaneous abortion within 20 weeks\' gestation, and 100 maintained pregnancy with the same gestational age.
METHODS: Vaginal swab was used to detect participants\' vaginal microbiota. DNA was extracted from the vaginal samples; the 16S ribosomal RNA sequencing method was used to identify the organisms present.
RESULTS: A pathogen detection rate of 13.15% (n = 200 women) was found in vaginal cultures. The percentages of bacterial detection in women who had spontaneous abortion and those whose pregnancies continued were 19.9% and 7.9%, respectively (p < .05). Compared to women who sustained their pregnancy, women who experienced spontaneous abortion had five common species: Escherichia coli (48%), Enterococcus faecalis (28%), Enterococcus faecium (12%), Pseudomonas fluorescens (7%), and Pseudomonas cedrina (5%); the most prevalent species among those with sustained pregnancy were Lactobacillus iners (58%), Lactobacillus crispatus (32 %), and E. coli (10%; p < .05).
CONCLUSIONS: The vaginal microbiomes of women with spontaneous abortion were more varied and irregularly distributed with bacteria. The bacteria identified could affect a woman\'s pregnancy outcome and perhaps be associated with spontaneous abortion. It is vital for nurses to provide education to women who are in the fertile period regarding prenatal screening, sexually transmitted infections, and perineal care.