在这项研究中,调查了摩洛哥LemnaminutaKunth自然栖息地的生态条件,以及五种合成生长培养基(Murashige-Skoog(MS),申克-希尔德布兰德(SH),霍格兰培养基(HM),10X藻类分析程序(AAP),和瑞典标准研究所培养基(SIS))对形态生理学和生化参数进行了分析。形态生理学参数包括根长,叶状体表面积,和新鲜的重量,而生化参数包括光合色素,碳水化合物,和蛋白质含量。该研究在体外分两个阶段进行:不受控制的曝气系统(阶段I)和受控的曝气系统(阶段II)。结果表明,电导率,盐度,自然栖息地的铵水平在浮萍生长的最佳范围内。测得的正磷酸盐浓度高于以前的观察结果,而记录的化学需氧量值较低。该研究还揭示了培养基组成对浮萍的形态生理学和生化参数的显着影响。新鲜的生物量,叶片的相对增长率,表面积的相对增长率,根长,蛋白质含量,碳水化合物,叶绿素(a),叶绿素(b),总叶绿素,类胡萝卜素,叶绿素(a/b)比值均受培养基的影响。最准确的回归模型描述了基于时间和两个阶段的体外培养条件的生长指数GI(F)。在第一阶段,MS的最佳型号,SIS,AAP,SH培养基是线性的,加权二次,立方,加权立方,分别。在第二阶段,所有生长培养基的最佳模型都是线性的.第二阶段的时间系数(以天为单位)为AAP的0.321、0.547、1.232、1.470和0.306,HM,MS,SH,SIS,分别。比较不同培养基叶片的形态生理和生化参数,并分析回归模型结果表明,在受控曝气条件下,SH和MS培养基是测试培养基中最佳的。然而,需要进一步研究以开发新的合成培养基,以最好地促进这种浮萍在长期培养中的生长和维持。
In this study, the ecological conditions of the natural habitat of Lemna minuta Kunth in Morocco were investigated, and the impact of five synthetic growth media (Murashige-Skoog (MS), Schenk-Hildebrand (SH), Hoagland medium (HM), 10X Algal Assay Procedure (AAP), and Swedish Standard Institute medium (SIS)) on the morphophysiological and biochemical parameters was analysed. The morphophysiological parameters included root length, frond surface area, and fresh weight, while the biochemical parameters included photosynthetic pigments, carbohydrates, and protein content. The study was conducted in vitro in two phases: an uncontrolled aeration system (Phase I) and a controlled aeration system (Phase II).The results showed that the pH, conductivity, salinity, and ammonium levels in the natural habitat were within the optimal range for duckweed growth. The measured orthophosphate concentrations were higher compared to previous observations, while the recorded chemical oxygen demand values were low. The study also revealed a significant effect of the culture medium composition on the morphophysiological and biochemical parameters of the duckweed. The fresh weight biomass, relative growth rate in fronds, relative growth rate in surface area, root length, protein content, carbohydrates, chlorophyll (a), chlorophyll (b), total chlorophyll, carotenoids, and the chlorophyll (a/b) ratio were all affected by the culture medium.The most accurate regression models described the growth index GI(F) based on time and in vitro culture conditions in both phases. In Phase I, the best models for MS, SIS, AAP, and SH media were linear, weighted quadratic, cubic, and weighted cubic, respectively. In Phase II, the best models for all growth media were linear. The time coefficients (in days) for Phase II were 0.321, 0.547, 1.232, 1.470, and 0.306 for AAP, HM, MS, SH, and SIS, respectively.Comparing the morphophysiological and biochemical parameters of fronds from different media and analysing the regression model results showed that the SH and MS media were the best among the tested media for the in vitro culture of L. minuta in controlled aeration conditions. However, further research is needed to develop new synthetic media that best promote the growth and maintenance of this duckweed in long-term culture.