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Abstract：
Successfully developed in 1976, the continuous in vitro culture of Plasmodium falciparum has many applications in the field of malaria research. It has become an important experimental model that directly uses a human pathogen responsible for a high prevalence of morbidity and mortality in many parts of the world and is a major source of biological material for immunological, biochemical, molecular, and pharmacological studies. Until present, the basic techniques described by Trager and Jensen and Haynes et al. remain unchanged in many malaria research laboratories. Nonetheless, different factors, including culture media, buffers, serum substitutes and supplements, sources of erythrocytes, and conditions of incubation (especially oxygen concentration), have been modified by different investigators to adapt the original technique in their laboratories or enhance the in vitro growth of the parasites. The possible effects and benefits of these modifications for the continuous cultivation of asexual intraerythrocytic stages of P. falciparum, as well as future challenges in developing a serum-free cultivation system and axenic cultures, are discussed.
摘要：
1976年研制成功的恶性疟原虫连续式体外培养在疟疾研究领域有许多应用。它已成为一个重要的实验模型，它直接使用人类病原体，在世界许多地方造成高发病率和死亡率，并且是免疫学生物材料的主要来源，生物化学，分子，和药理学研究。直到现在,Trager和Jensen和Haynes等人描述的基本技术。在许多疟疾研究实验室中保持不变。尽管如此，不同的因素,包括文化媒介,缓冲区,血清替代品和补充剂，红细胞来源，和培养条件（尤其是氧气浓度），已由不同的研究人员进行了修改，以适应实验室中的原始技术或增强寄生虫的体外生长。这些修饰对恶性疟原虫无性红细胞内阶段的连续培养的可能影响和益处，以及开发无血清培养系统和无菌培养的未来挑战，正在讨论。
