PDF(Pubmed)
Abstract：
The systematical characterization and understanding of the metabolic behaviors are the basis of the efficient plant metabolic engineering and synthetic biology. Genome-scale metabolic networks (GSMNs) are indispensable tools for the comprehensive characterization of overall metabolic profile. Here we first constructed a GSMN of tobacco, which is one of the most widely used plant chassis, and then combined the tobacco GSMN and multiomics analysis to systematically elucidate the impact of in-vitro cultivation on the tobacco metabolic network. In-vitro cultivation is a widely used technique for plant cultivation, not only in the field of basic research but also for the rapid propagation of valuable horticultural and pharmaceutical plants. However, the systemic effects of in-vitro cultivation on overall plant metabolism could easily be overlooked and are still poorly understood. We found that in-vitro tobacco showed slower growth, less biomass and suppressed photosynthesis than soil-grown tobacco. Many changes of metabolites and metabolic pathways between in-vitro and soil-grown tobacco plants were identified, which notably revealed a significant increase of the amino acids content under in-vitro condition. The in silico investigation showed that in-vitro tobacco downregulated photosynthesis and primary carbon metabolism, while significantly upregulated the GS/GOGAT cycle, as well as producing more energy and less NADH/NADPH to acclimate in-vitro growth demands. Altogether, the combination of experimental and in silico analyses offers an unprecedented view of tobacco metabolism, with valuable insights into the impact of in-vitro cultivation, enabling more efficient utilization of in-vitro techniques for plant propagation and metabolic engineering.
摘要：
对代谢行为的系统表征和理解是高效植物代谢工程和合成生物学的基础。基因组尺度的代谢网络（GSMN）是全面表征整体代谢谱不可或缺的工具。在这里，我们首先构建了烟草的GSMN，这是使用最广泛的工厂底盘之一，然后结合烟草GSMN和多组学分析，系统地阐明了离体培养对烟草代谢网络的影响。离体培养是一种广泛使用的植物栽培技术,不仅在基础研究领域，而且在有价值的园艺和药用植物的快速繁殖方面。然而,体外培养对植物整体代谢的系统性影响很容易被忽视，目前仍知之甚少。我们发现离体烟草生长较慢，与土壤种植的烟草相比，生物量较少，光合作用受到抑制。确定了体外和土壤种植的烟草植物之间代谢产物和代谢途径的许多变化，显着表明在体外条件下氨基酸含量显着增加。计算机研究表明，离体烟草下调光合作用和初级碳代谢，虽然显著上调GS/GOGAT周期，以及产生更多的能量和更少的NADH/NADPH来适应体外生长需求。总之,实验和计算机模拟分析的结合提供了前所未有的烟草代谢观点，对体外培养的影响有了宝贵的见解，能够更有效地利用体外技术进行植物繁殖和代谢工程。
