PDF(Pubmed)
Abstract：
Plant cryobanks play a significant role in modern science and breeding. They contribute to the recovery of lost species, the emergence of new plant varieties, and help preserve and explore the diversity of the plant world. The IPPRAS Cryobank collection is constantly supplemented with new samples, while, at the same time, the stored samples are being monitored. In order to test seed germination, seeds of Allium and Veratrum species were thawed. Rare Allium species seeds, such as A. nutans, A. schoenoprasum, and A. victorialis were stored in liquid nitrogen for 17, 19, and 30 years, respectively. Long-term cryopreservation decreased germination rates for A. nutans from 96.55 to 50.00%, for A. schoenoprasum from 72.00 to 62.75%, and for A. victorialis from 90.00 to 83.05%. Seeds of a rare medicinal species, Veratrum lobelianum, were stored in liquid nitrogen for 18 years; the seed germination rate during this storage period has been significantly decreased from 75.00 to 14.81%. V. nigrum seeds were also collected and frozen in liquid nitrogen for 3 days. Short-term cryopreservation did not result in a statistically significant change in germination rates (from 79.71 to 82.69%). The seeds of an endangered ornamental species, Cypripedium calceolus, were collected and kept frozen for 3 days. After cryopreservation, the seeds were planted on three different media, as follows: ½ MS, MS with 10% coconut milk, and BM1. On ½ MS medium, 24.98% seeds formed protocorms, while on MS medium with 10% coconut milk, this number was 10.02%, and on BM1 medium, it was 15.02%, respectively; however, after 2.5 months, all of the protocorms died. Thus, it appears that the existing protocol for seed cryopreservation of C. calceolus needs further improvement. The size, weight, and free water content (WC) of six previously cryopreserved Stipa species and three Allium species were measured. For all the Allium and Stipa species studied, we found no correlation between seed size, WC, and cryotolerance. We also found no correlation between the life form, which reflects the water requirement of the species, and cryotolerance.
摘要：
植物冷冻库在现代科学和育种中起着重要作用。它们有助于恢复丢失的物种，植物新品种的出现，并帮助保护和探索植物世界的多样性。IPPRASCryobank系列不断补充新样品，while,同时,存储的样品正在被监测。为了测试种子发芽，葱属和verratum物种的种子被解冻。稀有葱属物种种子，例如A.Nutans，A.学校规范，紫草在液氮中储存了17年、19年和30年，分别。长期冷冻保存可使坚果的发芽率从96.55降至50.00%，对于A.schoenoprasum从72.00到62.75%，维氏A从90.00到83.05%。一种稀有药用物种的种子，Veratumlobelianum,在液氮中保存了18年；在此存储期间，种子发芽率已从75.00％显着降低到14.81％。还收集黑曲霉种子并在液氮中冷冻3天。短期冷冻保存不会导致发芽率的统计学显着变化（从79.71到82.69％）。濒危观赏物种的种子，钙蓝,收集并冷冻3天。冷冻保存后,种子被种植在三种不同的培养基上，如下：½MS，MS含10％椰奶，BM1在½MS培养基上，24.98%的种子形成原球茎，在含有10％椰奶的MS培养基上，这个数字是10.02%,在BM1培养基上，是15.02%,分别；然而，2.5个月后，所有的原球虫都死了.因此，现有的黄云种子冷冻保存方案似乎需要进一步改进.大小,体重,并测量了六个先前冷冻保存的针茅属和三个葱属的自由水含量（WC）。对于所有研究的葱属和针茅物种，我们发现种子大小之间没有相关性，WC,和低温耐受性。我们还发现生命形式之间没有相关性，这反映了物种的需水量，和低温耐受性。
