PDF(Pubmed)
Abstract：
The cultivation of meat using in vitro grown animal stem cells offers a promising solution to pressing global concerns around climate change, ethical considerations, and public health. However, cultivated meat introduces an unprecedented necessity: the generation of mass scales of cellular biomaterial, achieved by fostering cell proliferation within bioreactors. Existing methods for in vitro cell proliferation encounter substantial challenges in terms of both scalability and economic viability. Within this perspective, we discuss the current landscape of cell proliferation optimization, focusing on approaches pertinent to cellular agriculture. We examine the mechanisms governing proliferation rates, while also addressing intrinsic and conditional rate limitations. Furthermore, we expound upon prospective strategies that could lead to a significant enhancement of the overall scalability and cost-efficiency of the cell proliferation phase within the cultivated meat production process. By exploring knowledge from basic cell cycle studies, pathological contexts and tissue engineering, we may identify innovative solutions toward optimizing cell expansion.
摘要：
使用体外培养的动物干细胞进行肉类培养为解决全球对气候变化的担忧提供了一个有希望的解决方案。伦理考虑，和公共卫生。然而,栽培肉引入了前所未有的必要性：大规模细胞生物材料的产生，通过促进生物反应器内的细胞增殖来实现。用于体外细胞增殖的现有方法在可扩展性和经济可行性方面都遇到了重大挑战。在这个视角下,我们讨论了细胞增殖优化的现状，专注于与细胞农业相关的方法。我们研究了控制扩散速率的机制，同时也解决了内在和有条件的利率限制。此外，我们阐述了前瞻性策略，这些策略可以显着提高栽培肉生产过程中细胞增殖阶段的总体可扩展性和成本效益。通过探索基本细胞周期研究的知识，病理背景和组织工程，我们可以确定创新的解决方案，以优化细胞扩增。
