■功能性无机纳米材料(NMs)被广泛用作生物活性材料和药物储库。在皮肤损伤部位缺乏稳定形式的NMs应用,可能会阻碍清创术的移除,提高pH值,诱导组织毒性,并限制它们在皮肤修复中的使用。这需要克服上述限制的创新伤口敷料的出现。这项研究的首要目的是利用锶掺杂的中孔硅颗粒(PSiSr)赋予基于聚(乳酸-羟基乙酸共聚物)/明胶(PG)的纤维敷料(PG@PSiSr)的多功能性,以进行切除伤口处理。
■使用化学合成方法合成了中孔硅颗粒(PSi)和PSiSr。使用静电纺丝将PSi和PSiSr两者结合到PG纤维中。一系列的结构,形态学,孔径分布,并对PG@PSi和PG@PSiSr膜进行了累积pH研究。细胞相容性,血液相容性,Transwell迁移,划痕伤口愈合,并在体外测试了这些复合敷料的血管生成特性。通过大鼠皮下植入模型评估复合敷料在体内的生物相容性,而通过在大鼠全层切除缺损模型中的植入可以识别它们的伤口愈合潜力。
■PG@PSiSr膜可以持续释放硅离子(Si4)和锶离子(Sr2)长达192小时,并显着促进人脐静脉内皮细胞(HUVEC)和NIH-3T3成纤维细胞的迁移。PG@PSiSr膜也显示出更好的细胞相容性,血液相容性,并在体外显著形成HUVECs的小管样网络。此外,PG@PSisr膜还促进宿主细胞的浸润并促进胶原蛋白的沉积,同时减少大鼠皮下植入模型中炎性细胞的积累,如评估的长达14天。在大鼠全层切除伤口模型中移植的膜的进一步评估显示伤口快速闭合(PG@SiSr与对照,96.1%vs71.7%),再上皮化,伴随皮肤附件形成的炎症反应较少(例如,血管,腺体,毛囊,等。).
■总而言之,我们成功地制备了PSisr颗粒,并使用静电纺丝制备了PG@PSisr敷料。PSiSr介导的治疗性离子释放,如Si4+和Sr2+,可以改善PLGA/凝胶敷料的功能,以进行有效的伤口修复,这也可能对其他软组织修复学科产生影响。
UNASSIGNED: Functional inorganic nanomaterials (NMs) are widely exploited as bioactive materials and drug depots. The lack of a stable form of application of NMs at the site of skin injury, may impede the removal of the debridement, elevate pH, induce tissue toxicity, and limit their use in skin repair. This necessitates the advent of innovative wound dressings that overcome the above limitations. The overarching objective of this study was to exploit strontium-doped mesoporous silicon particles (PSiSr) to impart multifunctionality to poly(lactic-co-glycolic acid)/gelatin (PG)-based fibrous dressings (PG@PSiSr) for excisional wound management.
UNASSIGNED: Mesoporous silicon particles (PSi) and PSiSr were synthesized using a chemo-synthetic approach. Both PSi and PSiSr were incorporated into PG fibers using electrospinning. A series of structure, morphology, pore size distribution, and cumulative pH studies on the PG@PSi and PG@PSiSr membranes were performed. Cytocompatibility, hemocompatibility, transwell migration, scratch wound healing, and delineated angiogenic properties of these composite dressings were tested in vitro. The biocompatibility of composite dressings in vivo was assessed by a subcutaneous implantation model of rats, while their potential for wound healing was discerned by implantation in a full-thickness excisional defect model of rats.
UNASSIGNED: The PG@PSiSr membranes can afford the sustained release of silicon ions (Si4+) and strontium ions (Sr2+) for up to 192 h as well as remarkably promote human umbilical vein endothelial cells (HUVECs) and NIH-3T3 fibroblasts migration. The PG@PSiSr membranes also showed better cytocompatibility, hemocompatibility, and significant formation of tubule-like networks of HUVECs in vitro. Moreover, PG@PSiSr membranes also facilitated the infiltration of host cells and promoted the deposition of collagen while reducing the accumulation of inflammatory cells in a subcutaneous implantation model in rats as assessed for up to day 14. Further evaluation of membranes transplanted in a full-thickness excisional wound model in rats showed rapid wound closure (PG@SiSr vs control, 96.1% vs 71.7%), re-epithelialization, and less inflammatory response alongside skin appendages formation (eg, blood vessels, glands, hair follicles, etc.).
UNASSIGNED: To sum up, we successfully fabricated PSiSr particles and prepared PG@PSiSr dressings using electrospinning. The PSiSr-mediated release of therapeutic ions, such as Si4+ and Sr2+, may improve the functionality of PLGA/Gel dressings for an effective wound repair, which may also have implications for the other soft tissue repair disciplines.