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Abstract：
This study aimed to develop a novel Gelatin silver oxide material for releasing nitric oxide bionanocomposite wound dressing with enhanced mechanical, chemical, and antibacterial properties for the treatment of diabetic wounds. The gelatin- silver oxide nanoparticles (Ag2O-NP) bio nanocomposite was prepared using chitosan and gelatin polymers incorporated with silver oxide nanoparticles through the freeze-drying method. The samples were characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis. Results showed that the Ag2O-NP nanoparticles increased porosity, decreased pore size, and improved elastic modulus. The Ag2O-NP wound dressing exhibited the most effective antibacterial properties against Staphylococcus aureus and Escherichia coli. Among the samples, the wound dressing containing silver oxide nanoparticles demonstrated superior physical and mechanical properties, with 48% porosity, a tensile strength of 3.2 MPa, and an elastic modulus of 51.7 MPa. The fabricated wound dressings had a volume ratio of empty space to total volume ranging from 40% to 60%. In parallel, considering the complications of diabetes and its impact on the vascular system, another aspect of the research focused on developing a per2mediated wound dressing capable of releasing nitric oxide gas to regenerate damaged vessels and accelerate diabetic wound healing. Chitosan, a biocompatible and biodegradable polymer, was selected as the substrate for the wound dressing, and beta-glycerophosphate (GPβ), tripolyphosphate (TPP), and per2mediated alginate (AL) were used as crosslinkers. The chitosan-alginate (CS-AL) wound dressing exhibited optimal characteristics in terms of hole count and uniformity in the scanning electron microscope test. It also demonstrated superior water absorption (3854%) and minimal air permeability. Furthermore, the CS-AL sample exhibited an 80% degradation rate after 14 days, indicating its suitability as a wound dressing. The wound dressing was loaded with S-nitrosoglutathione (GSNO) powder, and the successful release of nitric oxide gas was confirmed through the grease test, showing a peak at a wavelength of 540 nm. Subsequent investigations revealed that the treatment of human umbilical vein endothelial cells (HUVECs) with high glucose led to a decrease in the expression of PER2 and SIRT1, while the expression of PER2 increased, which may subsequently enhance the expression of SIRT1 and promote cell proliferation activity. However, upon treatment of the cells with the modified materials, an increase in the expression of PER2 and SIRT1 was observed, resulting in a partial restoration of cell proliferative activity. This comprehensive study successfully developed per2-mediated bio-nanocomposite wound dressings with improved physical, mechanical, chemical, and antibacterial properties. The incorporation of silver oxide nanoparticles enhanced the antimicrobial activity, while the released nitric oxide gas from the dressing demonstrated the ability to mitigate vascular endothelial cell damage induced by high glucose levels. These advancements show promising potential for facilitating the healing process of diabetic wounds by addressing complications associated with diabetes and enhancing overall wound healing.
摘要：
本研究旨在开发一种新型明胶氧化银材料，用于释放一氧化氮生物纳米复合伤口敷料，化学,和抗菌性能的糖尿病伤口的治疗。明胶-氧化银纳米颗粒（Ag2O-NP）生物纳米复合材料是使用壳聚糖和明胶聚合物与氧化银纳米颗粒通过冷冻干燥方法制备的。使用扫描电子显微镜（SEM）和X射线衍射（XRD）分析对样品进行了表征。结果表明，Ag2O-NP纳米颗粒增加了孔隙率，孔径减小，提高了弹性模量。Ag2O-NP伤口敷料对金黄色葡萄球菌和大肠杆菌表现出最有效的抗菌性能。在样本中，含有氧化银纳米颗粒的伤口敷料表现出优异的物理和机械性能，孔隙率为48%，抗拉强度为3.2MPa，弹性模量为51.7MPa。制造的伤口敷料的空空间与总体积的体积比在40%至60%的范围内。并行,考虑到糖尿病的并发症及其对血管系统的影响，研究的另一方面集中在开发一种能够释放一氧化氮气体以再生受损血管并加速糖尿病伤口愈合的全介导伤口敷料。壳聚糖,一种生物相容性和生物可降解的聚合物，被选为伤口敷料的基质，和β-甘油磷酸盐（GPβ），三聚磷酸盐（TPP），和过2介导的藻酸盐（AL）用作交联剂。在扫描电子显微镜测试中，壳聚糖-海藻酸盐（CS-AL）伤口敷料在孔数和均匀性方面表现出最佳特征。它还表现出优异的吸水率(3854%)和最小的透气性。此外，CS-AL样品在14天后表现出80%的降解率，表明其作为伤口敷料的适用性。伤口敷料装载有S-亚硝基谷胱甘肽（GSNO）粉末，通过油脂测试确认一氧化氮气体的成功释放，在540nm的波长处显示峰值。随后的研究表明，用高糖处理人脐静脉内皮细胞（HUVECs）导致PER2和SIRT1的表达降低，而PER2的表达增加，这可能随后增强SIRT1的表达并促进细胞增殖活性。然而,用改性材料处理细胞后，观察到PER2和SIRT1的表达增加，导致细胞增殖活性的部分恢复。这项综合研究成功开发了per2介导的生物纳米复合伤口敷料，机械,化学,和抗菌性能。氧化银纳米颗粒的掺入增强了抗菌活性，而从敷料释放的一氧化氮气体证明了减轻高葡萄糖水平引起的血管内皮细胞损伤的能力。这些进步显示出通过解决与糖尿病相关的并发症并增强整体伤口愈合来促进糖尿病伤口愈合过程的有希望的潜力。
