在植物中,授粉和受精过程中的花粉-雌蕊相互作用介导花粉水合和萌发,花粉管生长,和种子的建立和发展。细胞壁转化酶(CWINs)有助于为花粉发育提供碳水化合物;然而,它们在授粉和受精中的作用尚未得到很好的确立。在黄瓜(Cucumissativus)中,CsCWIN3在花朵中表达量最高,我们进一步检查了CsCWIN3在授粉到种子集时的功能。CsCWIN3转录物和CsCWIN3蛋白在萼片中表现出相似的表达模式,花瓣,雄蕊花丝,花药绒毡层,和雄花的花粉,以及在耻辱中,风格,发射道,雌花的胚珠。值得注意的是,黄瓜中CsCWIN3的抑制不会影响单性结实果实的形成,但会导致雌花中柱头外植体的生长停滞,花药的部分开裂延迟,雄花中花粉活力降低。因此,授粉后,花粉管在雌蕊中生长不良。此外,CsCWIN3-RNAi(RNA干扰)植物也显示受影响的种子发育。考虑到糖转运蛋白可以在黄瓜繁殖力中起作用,我们强调了CsCWIN3的作用以及CWIN和糖转运蛋白在这些过程中的潜在密切合作。总的来说,我们使用分子和生理分析来确定花粉形成过程中CsCWIN3介导的代谢,花粉管生长,和植物繁殖力。CsCWIN3在黄瓜中具有从授粉和受精到结实的重要作用,而不是单性结实的果实发育。
In plants, pollen-pistil interactions during pollination and fertilization mediate pollen hydration and germination, pollen tube growth, and seed set and development. Cell wall invertases (CWINs) help provide the carbohydrates for pollen development; however, their roles in pollination and fertilization have not been well established. In cucumber (Cucumis sativus), CsCWIN3 showed the highest expression in flowers, and we further examined CsCWIN3 for functions during pollination to seed set. Both CsCWIN3 transcript and CsCWIN3 protein exhibited similar expression patterns in the sepals, petals, stamen filaments, anther tapetum, and pollen of male flowers, as well as in the stigma, style, transmitting tract, and ovule funiculus of female flowers. Notably, repression of CsCWIN3 in cucumber did not affect the formation of parthenocarpic fruit but resulted in an arrested growth of stigma integuments in female flowers and a partially delayed dehiscence of anthers with decreased pollen viability in male flowers. Consequently, the pollen tube grew poorly in the gynoecia after pollination. In addition, CsCWIN3-RNA interference plants also showed affected seed development. Considering that sugar transporters could function in cucumber fecundity, we highlight the role of CsCWIN3 and a potential close collaboration between CWIN and sugar transporters in these processes. Overall, we used molecular and physiological analyses to determine the CsCWIN3-mediated metabolism during pollen formation, pollen tube growth, and plant fecundity. CsCWIN3 has essential roles from pollination and fertilization to seed set but not parthenocarpic fruit development in cucumber.