RESULTS: Glucose oxidase was successfully expressed and co-localized with β-fructofuranosidase in mycelia. These mycelia were applied to synthesis of FOS, which possessed an increased purity of 60.63% from 52.07%. Furthermore, peroxidase was expressed in A. niger and reached 7.70 U/g, which could remove the potential inhibitor of glucose oxidase to facilitate the FOS synthesis. Finally, the glucose oxidase-expressing strain and the peroxidase-expressing strain were jointly used to synthesize FOS, which content achieved 71.00%.
CONCLUSIONS: This strategy allows for obtaining high-content FOS by the multiple enzymes expressed in the industrial fungus, avoiding additional purification processes used in the production of oligosaccharides. This study not only facilitated the high-purity FOS synthesis, but also demonstrated the potential of A. niger ATCC 20611 as an enzyme-producing cell factory.
结果:葡萄糖氧化酶在菌丝体中成功表达并与β-呋喃果糖苷酶共定位。这些菌丝体被应用于FOS的合成,纯度从52.07%提高到60.63%。此外,过氧化物酶在黑曲霉中表达,达到7.70U/g,可以去除葡萄糖氧化酶的潜在抑制剂,促进FOS的合成。最后,葡萄糖氧化酶表达菌株和过氧化物酶表达菌株联合合成FOS,其含量达到71.00%。
结论:该策略允许通过在工业真菌中表达的多种酶获得高含量的FOS,避免在低聚糖的生产中使用额外的纯化过程。本研究不仅促进了高纯度FOS的合成,但也证明了黑曲霉ATCC20611作为产酶细胞工厂的潜力。