RESULTS: This study systematically analyzed the D. officinale genome and identified four DoAINV genes, which were classified into two subfamilies based on subcellular prediction and phylogenetic analysis. Comparison of gene structures and conserved motifs in DoAINV genes indicated a high-level conservation during their evolution history. The conserved amino acids and domains of DoAINV proteins were identified as pivotal for their functional roles. Additionally, cis-elements associated with responses to abiotic and biotic stress were found to be the most prevalent motif in all DoAINV genes, indicating their responsiveness to stress. Furthermore, bioinformatics analysis of transcriptome data, validated by quantitative real-time reverse transcription PCR (qRT-PCR), revealed distinct organ-specific expression patterns of DoAINV genes across various tissues and in response to abiotic stress. Examination of soluble sugar content and interaction networks provided insights into stress release and sucrose metabolism.
CONCLUSIONS: DoAINV genes are implicated in various activities including growth and development, stress response, and polysaccharide biosynthesis. These findings provide valuable insights into the AINV gene amily of D. officinale and will aid in further elucidating the functions of DoAINV genes.
结果:本研究系统分析了杜松子酒基因组,鉴定出4个DoAINV基因,根据亚细胞预测和系统发育分析将其分为两个亚家族。DoAINV基因的基因结构和保守基序的比较表明,在其进化史上具有高水平的保守性。DoAINV蛋白的保守氨基酸和结构域被鉴定为其功能作用的关键。此外,发现与对非生物和生物胁迫的反应相关的顺式元件是所有DoAINV基因中最普遍的基序,表明他们对压力的反应。此外,转录组数据的生物信息学分析,通过定量实时逆转录PCR(qRT-PCR)验证,揭示了DoAINV基因在各种组织中以及对非生物胁迫的不同器官特异性表达模式。对可溶性糖含量和相互作用网络的检查提供了对压力释放和蔗糖代谢的见解。
结论:DoAINV基因参与各种活动,包括生长和发育,应激反应,和多糖生物合成。这些发现提供了对D.officinaleAINV基因的宝贵见解,并将有助于进一步阐明DoAINV基因的功能。