%0 Journal Article
%T Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger.
%A Wan X
%A Wang L
%A Chang J
%A Zhang J
%A Zhang Z
%A Li K
%A Sun G
%A Liu C
%A Zhong Y
%J Microb Cell Fact
%V 23
%N 1
%D 2024 Mar 9
%M 38461254
%F 6.352
%R 10.1186/s12934-024-02353-w
%X <B>BACKGROUND: </B>Aspergillus niger ATCC 20611 is an industrially important fructooligosaccharides (FOS) producer since it produces the β-fructofuranosidase with superior transglycosylation activity, which is responsible for the conversion of sucrose to FOS accompanied by the by-product (glucose) generation. This study aims to consume glucose to enhance the content of FOS by heterologously expressing glucose oxidase and peroxidase in engineered A. niger.<BR><B>RESULTS: </B>Glucose oxidase was successfully expressed and co-localized with β-fructofuranosidase in mycelia. These mycelia were applied to synthesis of FOS, which possessed an increased purity of 60.63% from 52.07%. Furthermore, peroxidase was expressed in A. niger and reached 7.70 U/g, which could remove the potential inhibitor of glucose oxidase to facilitate the FOS synthesis. Finally, the glucose oxidase-expressing strain and the peroxidase-expressing strain were jointly used to synthesize FOS, which content achieved 71.00%.<BR><B>CONCLUSIONS: </B>This strategy allows for obtaining high-content FOS by the multiple enzymes expressed in the industrial fungus, avoiding additional purification processes used in the production of oligosaccharides. This study not only facilitated the high-purity FOS synthesis, but also demonstrated the potential of A. niger ATCC 20611 as an enzyme-producing cell factory.