我们使用基于DNA的焦磷酸测序技术来表征不同粪肥比例的苹果园沙质土壤的细菌群落结构。五个粪肥百分比(5%,10%,15%,20%和25%)进行了检查。对于每个重复获得超过10,000个有效读段。群落由五个优势群组成(变形杆菌,放线菌,氯氟菌,酸杆菌和拟杆菌),其中随着粪肥比例从0%增加到25%,变形杆菌含量从41.38%逐渐下降到37.29%,分别。冗余分析表明,37个类别与粪肥比例高度相关,其中18个呈正相关。聚类表明,根际样品分为三个部分:低肥料(对照,5%)处理,中等粪肥(10%,15%)处理和高肥(20%,25%)处理。维恩对这三组物种类型的分析表明,细菌群落差异主要反映在数量比而不是物种多样性上。尽管粪便含量增加导致土壤有机质含量增加,中肥改良土壤表现出最高的脲酶活性和糖酶活性,而5%至20%的粪肥比率提高也导致细菌多样性高于对照和25%的粪肥比率处理。我们的实验结果表明,使用适当的粪肥比例导致显著更高的土壤酶活性和不同的细菌群落模式,而使用过量的粪肥对土壤质量有负面影响。
We used DNA-based pyrosequencing to characterize the bacterial community structure of the sandy soil of an apple orchard with different manure ratios. Five manure percentages (5%, 10%, 15%, 20% and 25%) were examined. More than 10,000 valid reads were obtained for each replicate. The communities were composed of five dominant groups (Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria and Bacteroidetes), of which Proteobacteria content gradually decreased from 41.38% to 37.29% as manure ratio increased from 0% to 25%, respectively. Redundancy analysis showed that 37 classes were highly correlated with manure ratio, 18 of which were positively correlated. Clustering revealed that the rhizosphere samples were grouped into three components: low manure (control, 5%) treatment, medium manure (10%, 15%) treatment and high manure (20%, 25%) treatment. Venn analysis of species types of these three groups revealed that the bacteria community difference was primarily reflected by quantity ratio rather than species variety. Although greater manure content led to higher soil organic matter content, the medium manure improved soil showed the highest urease activity and saccharase activity, while 5% to 20% manure ratio improvement also resulted in higher bacteria diversity than control and 25% manure ratio treatment. Our experimental results suggest that the use of a proper manure ratio results in significantly higher soil enzyme activity and different bacteria community patterns, whereas the use of excessive manure amounts has negative effect on soil quality.