Abstract：
Phenylalanine (Phe) accelerates fruit wound healing by activating phenylpropanoid metabolism. However, whether Phe affects sucrose and respiratory metabolism in fruit during wound healing remains unknown. In this research, we found that preharvest Phe spray promoted sucrose degradation and increased glucose and fructose levels by activating acid invertase (AI), neutral invertase (NI), sucrose synthase (SS) and sucrose phosphate synthase (SPS) on harvested muskmelons. The spray also activated hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), malate dehydrogenase (MDH), succinate dehydrogenase (SDH) and glucose-6-phosphate dehydrogenase (G6PDH). In addition, the spray improved energy and reducing power levels in the fruit. Taken together, preharvest Phe spray can provide carbon skeleton, energy and reducing power for wound healing by activating the sucrose metabolism, Embden-Meyerhof-Parnas (EMP) pathway, tricarboxylic acid (TCA) cycle and pentose phosphate (PPP) pathway in muskmelon wounds during healing, which is expected to be developed as a new strategy to accelerate fruit wound healing.
摘要：
苯丙氨酸（Phe）通过激活类苯丙素代谢加速果实伤口愈合。然而,在伤口愈合过程中，Phe是否会影响水果中的蔗糖和呼吸代谢，目前尚不清楚。在这项研究中,我们发现收获前Phe喷雾通过激活酸性转化酶（AI）促进蔗糖降解并增加葡萄糖和果糖水平，中性转化酶（NI），收获的甜瓜上的蔗糖合酶（SS）和蔗糖磷酸合酶（SPS）。喷雾还激活己糖激酶（HK），磷酸果糖激酶（PFK），丙酮酸激酶（PK），苹果酸脱氢酶（MDH），琥珀酸脱氢酶（SDH）和葡萄糖-6-磷酸脱氢酶（G6PDH）。此外,喷雾提高了能量并降低了水果中的功率水平。一起来看,收获前Phe喷雾可以提供碳骨架，能量和通过激活蔗糖代谢减少伤口愈合的力量，Embden-Meyerhof-Parnas(EMP)通路，甜瓜伤口愈合过程中的三羧酸（TCA）循环和磷酸戊糖（PPP）途径，有望作为加速水果伤口愈合的新策略。
