{Reference Type}: Journal Article
{Title}: Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger.
{Author}: Wan X;Wang L;Chang J;Zhang J;Zhang Z;Li K;Sun G;Liu C;Zhong Y;
{Journal}: Microb Cell Fact
{Volume}: 23
{Issue}: 1
{Year}: 2024 Mar 9
{Factor}: 6.352
{DOI}: 10.1186/s12934-024-02353-w
{Abstract}: <B>BACKGROUND: </B>Aspergillus niger ATCC 20611 is an industrially important fructooligosaccharides (FOS) producer since it produces the β-fructofuranosidase with superior transglycosylation activity, which is responsible for the conversion of sucrose to FOS accompanied by the by-product (glucose) generation. This study aims to consume glucose to enhance the content of FOS by heterologously expressing glucose oxidase and peroxidase in engineered A. niger.<BR><B>RESULTS: </B>Glucose oxidase was successfully expressed and co-localized with β-fructofuranosidase in mycelia. These mycelia were applied to synthesis of FOS, which possessed an increased purity of 60.63% from 52.07%. Furthermore, peroxidase was expressed in A. niger and reached 7.70 U/g, which could remove the potential inhibitor of glucose oxidase to facilitate the FOS synthesis. Finally, the glucose oxidase-expressing strain and the peroxidase-expressing strain were jointly used to synthesize FOS, which content achieved 71.00%.<BR><B>CONCLUSIONS: </B>This strategy allows for obtaining high-content FOS by the multiple enzymes expressed in the industrial fungus, avoiding additional purification processes used in the production of oligosaccharides. This study not only facilitated the high-purity FOS synthesis, but also demonstrated the potential of A. niger ATCC 20611 as an enzyme-producing cell factory.