目的:尽管存在一些局限性,但在过去的几十年中,氢氧化钙(CH)一直被认为是直接制浆材料(DPC)。与CH(CHPPL)结合的磷酸支链淀粉(PPL)是一种新型生物材料,被作为有前途的DPC材料引入。因此,该研究的目的是评估基于PPL的CH制剂对DPC后大鼠磨牙的炎症反应和矿化组织形成(MTF)能力。
方法:本研究由六组组成:CH+1%PPL(CHPPL-1);3%PPL(CHPPL-3);5%PPL(CHPPL-5);Dycal和NEXMTA骨水泥(N-MTA)作为阳性对照,和没有封盖材料(NC)。在Wistar大鼠上制备了120个上颌第一磨牙腔。封顶后,所有空洞均用4-META/MMA-TBB树脂修复,并在第1,7和28天评估牙髓反应.进行Kruskal-Wallis随后的Mann-WhitneyU检验,显著性水平为0.05。IL-6、Nestin、观察到DMP-1。
结果:在第1天,CHPPL-1,N-MTA,Dycal没有表现出轻度炎症,而CHPPL-3,CHPPL-5和NC显示轻度至中度炎症,结果有显著性差异(p<0.05)。在第7天,在CHPPL-1,N-MTA中观察到轻度至中度炎症,和Dycal,而CHPPL-3,CHPPL-5和NC表现出中度至重度炎症。CHPPL-1和N-MTA与NC之间存在显着差异(p<0.05),CHPPL-1和CHPPL-3与CHPPL-5和Dycal(p<0.05),和CHPPL-3与N-MTA(p<0.05)。在所有组中观察到矿化组织形成(MTF)的薄层。在第28天,CHPPL-1,Dycal,N-MTA显示无轻度炎症,而CHPPL-3,CHPPL-5和NC表现出轻度至重度炎症,差异有统计学意义(p<0.05)。CHPPL-1,Dycal,N-MTA表现出连续的MTF,而CHPPL-3,CHPPL-5和NC的MTF较厚且中断。CHPPL-1,CHPPL-3和N-MTA与NC组之间存在显着差异(p<0.05)。IL-6、Nestin、和DMP-1表明材料对成牙本质细胞样细胞形成和组织矿化的影响存在差异。
结论:这些研究结果表明,CHPPL-1具有减少牙髓炎症和促进MTF的潜力,并且具有与MTA骨水泥相似的功效。
OBJECTIVE: Calcium hydroxide (CH) has been considered as a direct pulp capping materials (DPC) for the last decades despite having some limitations. Phosphorylate pullulan (PPL) incorporated with CH (CHPPL) is a novel biomaterial that was introduced as a promising DPC material. Thus, the aim of the
study was to evaluate the inflammatory response and mineralized tissue formation (MTF) ability of PPL-based CH formulations on rat molars after DPC.
METHODS: This
study consisted of six groups: CH with 1% PPL (CHPPL-1); 3% PPL (CHPPL-3); 5% PPL (CHPPL-5); Dycal and NEX MTA Cement (N-MTA) as the positive control, and no capping materials (NC). One hundred twenty maxillary first molar cavities were prepared on Wistar rats. After capping, all the cavities were restored with 4-META/MMA-TBB resin and pulpal responses were evaluated at days 1, 7, and 28. Kruskal-Wallis followed by Mann-Whitney U-test was performed with a significance level of 0.05. Immunohistochemical expression of IL-6, Nestin, and DMP-1 was observed.
RESULTS: At day 1, CHPPL-1, N-MTA, and Dycal exhibited no to mild inflammation, whilst CHPPL-3, CHPPL-5, and NC showed mild to moderate inflammation, and the results were significantly different (p < .05). At day 7, mild to moderate inflammation was observed in CHPPL-1, N-MTA, and Dycal, whereas CHPPL-3, CHPPL-5, and NC exhibited moderate to severe inflammation. Significant differences were observed between CHPPL-1 and N-MTA with NC (p < .05), CHPPL-1 and CHPPL-3 with CHPPL-5 and Dycal (p < .05), and CHPPL-3 with N-MTA (p < .05). A thin layer of mineralized tissue formation (MTF) was observed in all groups. At day 28, CHPPL-1, Dycal, and N-MTA showed no to mild inflammation, whilst CHPPL-3, CHPPL-5, and NC exhibited mild to severe inflammation, and statistically significant difference was detected (p < .05). CHPPL-1, Dycal, and N-MTA exhibited continuous MTF, whilst CHPPL-3, CHPPL-5, and NC had thicker and interrupted MTF. Significant differences were observed between CHPPL-1, CHPPL-3, and N-MTA with NC group (p < .05). Variable expressions of IL-6, Nestin, and DMP-1 indicated differences in the materials\' impact on odontoblast-like cell formation and tissue mineralization.
CONCLUSIONS: These findings suggest that CHPPL-1 has the potential to minimize pulpal inflammation and promote MTF and had similar efficacy as MTA cement.