Abstract：
BACKGROUND: The repair process of periradicular tissues depends, among other factors, on the properties of endodontic cements. One of the main cells involved in this process are macrophages.
METHODS: Murine peritoneal macrophages obtained from C57BL/6 (MBL6) and BALB/c (MBalb) mice, respectively, were cultured with capillaries containing or not Endosequence BC Sealer® (BC), Sealer Plus BC® (MK), Bio C Sealer (Ang) and MTA®. Cell viability was measured by trypan blue and MTT methods at 24, 48 and 72 hours. Cell adhesion, phagocytosis of S. boulardii, production of reactive oxygen species (ROS), nitric oxide (NO), and the cytokines TNF-α and TGF-β, were also evaluated. The data were analysed using the ANOVA test (p<0.05).
RESULTS: Cell viability was similar between bioceramic sealers and MTA (p>0.05). There was no statistical difference between both macrophages when adherence and phagocytose were assayed. The presence of inflammation stimulus significantly altered the production of ROS by MBL6 macrophages in contact with the cements. The production of TGF-β was similar for both lineages of macrophages.
CONCLUSIONS: This study shows that the evaluated bioceramic cements do not interfere with MBL6 and MBalb macrophages adhesion, phagocytic capacity, as well as TGF-β production. The cements stimulated the production of ROS by MBL6 macrophages in response to induced inflammation, potentially favouring the elimination of residual pathogens.
METHODS: Murine peritoneal macrophages obtained from C57BL/6 (MBL6) and BALB/c (MBalb) mice, respectively, were cultured with capillaries containing or not Endosequence BC Sealer® (BC), Sealer Plus BC® (MK), Bio C Sealer (Ang) and MTA®. Cell viability was measured by trypan blue and MTT methods at 24, 48 and 72 hours. Cell adhesion, phagocytosis of S. boulardii, production of reactive oxygen species (ROS), nitric oxide (NO), and the cytokines TNF-α and TGF-β, were also evaluated. The data were analysed using the ANOVA test (p<0.05).
RESULTS: Cell viability was similar between bioceramic sealers and MTA (p>0.05). There was no statistical difference between both macrophages when adherence and phagocytose were assayed. The presence of inflammation stimulus significantly altered the production of ROS by MBL6 macrophages in contact with the cements. The production of TGF-β was similar for both lineages of macrophages.
CONCLUSIONS: This study shows that the evaluated bioceramic cements do not interfere with MBL6 and MBalb macrophages adhesion, phagocytic capacity, as well as TGF-β production. The cements stimulated the production of ROS by MBL6 macrophages in response to induced inflammation, potentially favouring the elimination of residual pathogens.
摘要：
背景：根尖周组织的修复过程取决于，在其他因素中，关于牙髓水泥的性质。参与该过程的主要细胞之一是巨噬细胞。
方法：从C57BL/6（MBL6）和BALB/c（MBalb）小鼠获得的小鼠腹膜巨噬细胞，分别,用含有或不含有EnnofsequenceBCSealer®(BC)的毛细血管培养，SealerPlusBC®(MK),BioC封口机(Ang)和MTA®。在24、48和72小时通过锥虫蓝和MTT方法测量细胞活力。细胞粘附,布拉氏链球菌的吞噬作用，活性氧（ROS）的产生，一氧化氮（NO），和细胞因子TNF-α和TGF-β，也进行了评估。使用ANOVA检验分析数据(p<0.05)。
结果:生物陶瓷密封剂和MTA之间的细胞活力相似(p>0.05)。当分析粘附性和吞噬作用时，两种巨噬细胞之间没有统计学差异。炎症刺激的存在显着改变了与水泥接触的MBL6巨噬细胞的ROS产生。两种巨噬细胞谱系的TGF-β的产生相似。
结论：这项研究表明，评估的生物陶瓷水泥不会干扰MBL6和MBalb巨噬细胞的粘附，吞噬能力，以及TGF-β的产生。粘固剂刺激MBL6巨噬细胞对诱导的炎症反应产生ROS，可能有利于消除残留的病原体。
方法：从C57BL/6（MBL6）和BALB/c（MBalb）小鼠获得的小鼠腹膜巨噬细胞，分别,用含有或不含有EnnofsequenceBCSealer®(BC)的毛细血管培养，SealerPlusBC®(MK),BioC封口机(Ang)和MTA®。在24、48和72小时通过锥虫蓝和MTT方法测量细胞活力。细胞粘附,布拉氏链球菌的吞噬作用，活性氧（ROS）的产生，一氧化氮（NO），和细胞因子TNF-α和TGF-β，也进行了评估。使用ANOVA检验分析数据(p<0.05)。
结果:生物陶瓷密封剂和MTA之间的细胞活力相似(p>0.05)。当分析粘附性和吞噬作用时，两种巨噬细胞之间没有统计学差异。炎症刺激的存在显着改变了与水泥接触的MBL6巨噬细胞的ROS产生。两种巨噬细胞谱系的TGF-β的产生相似。
结论：这项研究表明，评估的生物陶瓷水泥不会干扰MBL6和MBalb巨噬细胞的粘附，吞噬能力，以及TGF-β的产生。粘固剂刺激MBL6巨噬细胞对诱导的炎症反应产生ROS，可能有利于消除残留的病原体。
