Carbon dots (CDs) are quasi-spherical carbon nanoparticles with excellent photoluminescence, good biocompatibility, favorable photostability, and easily modifiable surfaces. CDs, serving as fluorescent probes, have emerged as an ideal tool for cellular differentiation owing to their outstanding luminescence performance and tunable surface properties. In this review, we summarize the recent research progress with CDs in the differentiation of cancer/normal cells, Gram-positive/Gram-negative bacteria, and live/dead cells, as well as the cellular differences used for differentiation. Additionally, we summarize the preparation methods, raw materials, and properties of the CDs used for cell discrimination. The differentiation mechanisms and the advantages or limitations of the differentiation methods are also introduced. Finally, we propose several research challenges in this field and future research directions that require extensive investigation. It is hoped that this review will help researchers in the design of new CDs as ideal fluorescent probes for realizing diverse cell differentiation applications.

Early disease detection is crucial since it raises the likelihood of treatment and considerably lowers the cost of therapy. Therefore, the improvement of human life and health depends on the development of quick, efficient, and credible biosensing methods. For improving the quality of biosensors, distinct nanostructures have been investigated; among these, carbon dots have gained much interest because of their great performance. Carbon dots, the essential component of fluorescence nanoparticles, having outstanding chemical characteristics, superb biocompatibility, chemical inertness, low toxicity and potential optical characteristics have attracted the researchers from every corner of the globe. Several carbon dots applications have been thoroughly investigated in recent decade, from optoelectronics to biomedical investigations. This review study primarily emphasizes the recent advancements in the field of biomass-derived carbon dots-based drug delivery, gene delivery and bioimaging, and highlights achievements in two major areas: in vivo applications that involve carbon dots absorption in zebrafish and mice, tumour therapeutics, and imaging-guided drug delivery. Additionally, the possible advantages, difficulties, and future possibilities of using carbon dots for biological applications are also explored.

Carbon dots (CDs) are a novel category of carbon-based nanomaterials characterized by their small size, often less than 10 nm. CDs physical, chemical, and optical properties can be tuned using one-pot assembly. Because of their non-toxicity, biocompatibility, chemical and physical responsiveness, photo- and chemical-bleaching resistance, and low cost, nanoparticles have become incredibly versatile. They find various applications in detecting inorganic substances, bio sensing, visualizing cells, studying biological processes in live cells, and aiding in medication delivery. Additionally, CDs exhibit versatility in electronics and energy storage, making them promising candidates for applications in solar cells, light-emitting diodes, and supercapacitors. CDs are more photo stable for hours than typical fluorescent semiconductor quantum dots. Before applying CDs, they must be characterized. Techniques such as UV-VIS spectroscopy, fluorescence spectrophotometry, FT-IR, TEM, XRD, Raman spectroscopy, and NMR are commonly used to assess their photophysical and structural properties. This article review explores the synthesis, characterization applications of CDs in analytical techniques for the determination of various analytes. The article provides a detailed analysis of the different methodologies used to make nanomaterials and devices for the characterization of CDs. It also discusses the challenges that arise when using CDs in analytical techniques for detecting different analytes. The focus of this review is on accurately determining pharmaceutical and biological samples using CDs as sensing probes.

As water pollution and scarcity pose severe threats to the sustainable progress of human society, it is important to develop a method or materials that can accurately and efficiently detect pollutants and purify aquatic environments or exploit marine resources. The compositing of photoluminescent and hydrophilic carbon dots (CDs) with hydrogels bearing three-dimensional networks to form CD-hydrogel composites to protect aquatic environments is a \"win-win\" strategy. Herein, the feasibility of the aforementioned method has been demonstrated. This paper reviews the recent progress of CD-hydrogel materials used in aquatic environments. First, the synthesis methods for these composites are discussed, and then, the composites are categorized according to different methods of combining the raw materials. Thereafter, the progress in research on CD-hydrogel materials in the field of water quality detection and purification is reviewed in terms of the application of the mechanisms. Finally, the current challenges and prospects of CD-hydrogel materials are described. These results are expected to provide insights into the development of CD-hydrogel composites for researchers in this field.

Biofilms are an intricate community of microbes that colonize solid surfaces, communicating via a quorum-sensing mechanism. These microbial aggregates secrete exopolysaccharides facilitating adhesion and conferring resistance to drugs and antimicrobial agents. The escalating global concern over biofilm-related infections on medical devices underscores the severe threat to human health. Carbon dots (CDs) have emerged as a promising substrate to combat microbes and disrupt biofilm matrices. Their numerous advantages such as facile surface functionalization and specific antimicrobial properties, position them as innovative anti-biofilm agents. Due to their minuscule size, CDs can penetrate microbial cells, inhibiting growth via cytoplasmic leakage, reactive oxygen species (ROS) generation, and genetic material fragmentation. Research has demonstrated the efficacy of CDs in inhibiting biofilms formed by key pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Consequently, the development of CD-based coatings and hydrogels holds promise for eradicating biofilm formation, thereby enhancing treatment efficacy, reducing clinical expenses, and minimizing the need for implant revision surgeries. This review provides insights into the mechanisms of biofilm formation on implants, surveys major biofilm-forming pathogens and associated infections, and specifically highlights the anti-biofilm properties of CDs emphasizing their potential as coatings on medical implants.

Detecting environmental contaminants is crucial for protecting ecosystems and human health. While traditional carbon dot (CD) fluorescent probes are versatile, they may suffer from limitations like fluctuations in signal intensity, leading to detection inaccuracies. In contrast, ratiometric fluorescent probes, designed with internal self-calibration mechanisms, offer enhanced sensitivity and reliability. This review focuses on the design and applications of ratiometric fluorescent probes based on CDs for environmental monitoring. Our discussion covers construction strategies, ratiometric fluorescence principles, and applications in detecting various environmental contaminants, including organic pollutants, heavy metal ions, and other substances. We also explore associated advantages and challenges and provide insights into potential solutions and future research directions.

The development of new nanocomposites has a significant impact on modern instrumentation and analytical methods for chemical analysis. Due to their unique properties, carbon dots (CDs) and silver nanoparticles (AgNPs), distinguished by their unique physical, electrochemical, and optical properties, have captivated significant attention. Thus, combining AgNPs and CDs may produce Ag/CDs nanocomposites with improved performances than the individual material. This comprehensive review offers an in-depth exploration of the synthesis, formation mechanism, properties, and the recent surge in chemical and biological sensing applications of Ag/CDs with their sensing mechanisms. Detailed insights into synthesis methods to produce Ag/CDs are unveiled, followed by information on their physicochemical and optical properties. The crux of this review lies in its spotlight on the diverse landscape of chemical and biological sensing applications of Ag/CDs, with a particular focus on fluorescence, electrochemical, colorimetric, surface-enhanced Raman spectroscopy, and surface plasmon resonance sensing techniques. The elucidation of sensing mechanisms of the nanocomposites with various target analytes adds depth to the discussion. Finally, this review culminates with a concise summary and a glimpse into future perspectives of Ag/CDs aiming to achieve highly efficient and enduring Ag/CDs for various applications.

Carbon nanoparticles (CDs) have recently drawn a great attention in (bio)chemical analysis, sensing and bioimaging owing to their photostability, water stability, minimal toxicity, biocompatibility and ease of surface functionalization. While the vast majority of CDs applications rely on exploiting their fluorescent properties, doping such nanomaterials with various elements has recently received increasing attention as an effective approach to modify their optoelectronic characteristics, introducing novel improved optical features such as phosphorescence, upconversion luminescence or multimodal imaging capabilities. This review article focuses in the recent advances on the synthesis of heteroatom-doped CDs, exhibiting distinctive features of high value for sensing and imaging, as well as various functionalization schemes developed for guided analyte labeling. Relevant applications in chemical sensing, bioimaging and disease therapy are here presented. A final section intends to provide an overview towards future developments of such emerging light-emitting nanomaterials in the design of future devices and strategies for (bio)analytical chemistry.

UNASSIGNED: Nanotechnology may open up new avenues for overcoming the challenges of pancreatic cancer therapy as a broad arsenal of anticancer medicines fail to realize their full therapeutic potential in pancreatic ductal adenocarcinoma due to the formation of multiple resistance mechanisms inside the tumor. Many studies have reported the successful use of various nano formulations in pancreatic cancer therapy.
UNASSIGNED: This review covers all the major nanotechnology-based patent litrature available on renowned patent data bases like Patentscope and Espacenet, through the time period of 2007-2022. This is an entirely patent centric review, and it includes both clinical and non-clinical data available on nanotechnology-based therapeutics and diagnostic tools for pancreatic cancer.
UNASSIGNED: For the sake of understanding, the patents are categorized under various formulation-specific heads like metallic/non-metallic nanoparticles, polymeric nanoparticles, liposomes, carbon nanotubes, protein nanoparticles and liposomes. This distinguishes one specific nanoparticle type from another and makes this review a one-of-a-kind comprehensive patent compilation that has not been reported so far in the history of nanotechnological formulations in pancreatic cancer.

Carbon dots (CDs) are nanomaterials with excellent properties, including good biocompatibility, small size, ideal photoluminescence and surface modification, and are becoming one of the most attractive nanomaterials for the imaging, detection and treatment of tumors. Based on these advantages, CDs can be combined other materials to obtain composite particles with improved, even new, performance, mainly in photothermal and photodynamic therapies. This paper reviews the research progress of CDs and their composites in targeted tumor imaging, detection, diagnosis, drug delivery and tumor killing. It also discusses and proposes the challenges and perspectives of their future applications in these fields. This review provides ideas for future applications of novel CD-based materials in the diagnosis and treatment of cancer.