目的:测定常规疫苗接种对健康马血清淀粉样蛋白A(SAA)浓度的影响。我们假设常规疫苗接种会增加健康马的SAA。
方法:21个明显健康的客户拥有的马和15个堪萨斯州立大学兽医学院拥有的马。
方法:在实验1(n=8匹马)中,一个盲人,随机化,prospective,进行交叉研究。马匹要么接种了疫苗(狂犬病,破伤风,西尼罗河,东部和西部马脑脊髓炎,马疱疹病毒-1/-4,流感)或生理盐水,SAA在第6、12和24小时测量,每天使用商业侧流免疫测定法直到第10天。在实验2中(n=28匹马),一个潜在的,观察性研究在疫苗接种后12小时和24小时以及每天直到第10天测量SAA。随时间重复测量的线性混合效应模型被马阻断,测试了治疗对SAA的影响。重复测量相关性测试了SAA与温度之间的相关性。
结果:随着时间的推移,与未接种疫苗的马相比,接种疫苗的马具有增加的模型调整的SAA,而没有不良反应的临床证据(P<0.01)。在实验1中,疫苗接种后模型调整的SAA在第2天达到峰值(中位数,1,872µg/mL;IQR,1,220.8至2,402.5µg/mL),并在第9天恢复正常(<20µg/mL)(中位数,6µg/mL;IQR,0.8至23.5µg/mL)接种后。在实验2中,接种疫苗的马具有随时间增加的SAA;温度和SAA不相关(P=0.78)。
结论:这项研究的结果表明,常规疫苗接种导致SAA浓度增加,并为疫苗接种后的恢复期提供了证据。疫苗接种后10天测量SAA不能用作疾病的指标。
OBJECTIVE: To measure the effect of routine vaccination on serum amyloid A (SAA) concentration in apparently healthy horses. We hypothesized that routine vaccination would increase SAA in healthy horses.
METHODS: 21 apparently healthy client-owned horses and 15 Kansas State University College of Veterinary Medicine-owned horses.
METHODS: In experiment 1 (n = 8 horses), a blinded, randomized, prospective, crossover study was performed. Horses were either vaccinated (rabies, tetanus, West Nile, Eastern and Western equine encephalomyelitis, equine herpesvirus-1/-4, influenza) or administered saline, and SAA was measured at 6, 12, and 24 hours and daily until day 10 with a commercial lateral-flow immunoassay. In experiment 2 (n = 28 horses), a prospective, observational study measured SAA after vaccination at 12 and 24 hours and daily until day 10. A linear mixed-effect model with repeated measures over time blocked by horse tested the effect of treatment on SAA. A repeated-measures correlation tested the correlation between SAA and temperature.
RESULTS: Over time, vaccinated horses had increased model-adjusted SAA compared to unvaccinated horses without clinical evidence of adverse reaction (P < .01). In experiment 1, the model-adjusted SAA after vaccination peaked on day 2 (median, 1,872 µg/mL; IQR, 1,220.8 to 2,402.5 µg/mL) and returned to normal (< 20 µg/mL) by day 9 (median, 6 µg/mL; IQR, 0.8 to 23.5 µg/mL) after vaccination. In experiment 2, vaccinated horses had increased SAA over time; temperature and SAA were not correlated (P = .78).
CONCLUSIONS: Results of this study indicated that routine vaccination results in increased SAA concentration and provided evidence for a period of convalescence following vaccination. Measuring SAA for 10 days following vaccination cannot be used as an indicator of illness.