Abstract：
Many studies have shown a strong correlation between Hindgut Acidosis (HGA) and the occurrence of laminitis in horses; therefore, the early diagnosis of HGA is essential. In this study, we investigated changes in the plasma concentrations of lipopolysaccharide-binding protein (LBP) and serum amyloid A (SAA) as inflammatory markers in horses with laminitis. Sixteen healthy male Arabian horses that had cecal cannulation without visible laminitis or general symptoms were randomly divided into two groups. The horses were fed two different diets in a forage-to-concentrate ratio. Blood samples were collected on Days 1, 10, and 20. The primary objective of this study was to analyze plasma levels of LBP and SAA. Cecal specimens were obtained from each equine subject on three designated days: days 1, 10, and 20. The second objective was to assess the levels of pH and volatile fatty acids (VFA) in the samples. Throughout the study period, horses fed a high-concentrate diet exhibited a significantly elevated average lameness grade on days 10 and 20 compared to the initial stage (P < 0.001). On day 20, a significant increase in the concentration of SAA was observed in horses fed a high-concentrate diet, in contrast to the initial stage of the study. LBP levels in the plasma were significantly elevated on days 10 and 20 in horses fed a high-concentrate diet. Based on our findings, it is recommended that the evaluation of plasma LBP concentrations is more effective than SAA for the early identification of HGA in horses fed a high-grain diet.
摘要：
许多研究表明，印肠酸中毒（HGA）与马的椎板炎之间存在很强的相关性；因此，HGA的早期诊断至关重要。在这项研究中,我们调查了患有椎板炎的马的血浆脂多糖结合蛋白(LBP)和血清淀粉样蛋白A(SAA)作为炎症标志物的浓度变化.16匹健康雄性阿拉伯马，盲肠插管无可见椎板炎或一般症状，随机分为两组。马匹以饲料与浓缩物的比例饲喂两种不同的饮食。在第1、10和20天收集血样。这项研究的主要目的是分析LBP和SAA的血浆水平。在指定的3天(第1、10和20天)从每个马受试者获得盲肠标本。第二个目的是评估样品中pH和挥发性脂肪酸(VFA)的水平。在整个研究期间，与初始阶段相比，饲喂高浓度饮食的马在第10天和第20天的平均跛行等级显着提高（P<0.001）。在第20天，在饲喂高浓缩饮食的马中观察到SAA浓度的显着增加，与研究的初始阶段相反。在第10天和第20天，饲喂高浓缩饮食的马的血浆中LBP水平显着升高。根据我们的发现,建议血浆LBP浓度的评估比SAA更有效地早期识别高谷物饮食的马的HGA。
