背景:无法解释的不孕症被定义为在无保护的性交1年后无法怀孕的夫妇进行的基本评估中没有任何病理。检查不孕症原因的测试结果显示,近15%的夫妇没有可识别的原因。
目的:本研究的目的是研究活性氧(ROS)对妊娠和胚胎的影响。
方法:这项研究包括200名患者,年龄在20-44岁之间,无法解释的不孕症,反复宫腔内人工授精失败,因此开始进行体外受精(IVF)/卵胞浆内单精子注射治疗。胚胎学家在取卵过程中从这些患者的卵母细胞中收集了一些废弃的卵泡液样品。接下来,总抗氧化剂状态(TAS),总氧化剂状态(TOS),和氧化应激指数(OSI)值在生物化学实验室计算。
结果:就怀孕状况而言,生化和临床妊娠患者的卵泡TOS和OSI值没有显着差异,而妊娠患者的TAS值明显高于妊娠患者(P<0.05)。就胚胎质量而言,在TAS中没有观察到显著差异,TOS,和OSI值在1级和2级胚胎之间,而接受1级胚胎移植的患者的妊娠率显着较高(P<0.05)。然而,吸烟患者的卵泡液TAS水平显著低于不吸烟患者;TOS和OSI水平显著高于不吸烟患者.
结论:这项研究表明,暴露于氧化应激可能是不孕症的一个致病因素。此外,ROS通过增加卵泡液中的OSI来降低TAS的水平;因此,抗氧化剂补充可能是必要的。
BACKGROUND: Unexplained infertility is defined as the absence of any pathology in the basic evaluation performed in couples who cannot achieve pregnancy after 1 year of unprotected sexual intercourse. The results of tests examining the causes of infertility show no identifiable cause in almost 15% of couples.
OBJECTIVE: The aim of this study was to investigate the effects of reactive oxygen species (ROS) on pregnancy and embryos.
METHODS: This study included 200 patients, aged between 20-44 years, with unexplained infertility, who had recurrent intrauterine inseminations failures and hence started in vitro fertilization (IVF)/intracytoplasmic sperm injection treatment. Some amounts of waste follicular fluid samples were collected by embryologists from the oocytes of these patients during the ovum pick-up procedure. Next, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values were calculated in the biochemistry laboratory.
RESULTS: In terms of pregnancy status, both follicular TOS and OSI values were not significantly different in patients with biochemical and clinical pregnancy, whereas TAS values were significantly higher in patients with pregnancy (P < 0.05). In terms of embryo quality, no significant difference was observed in TAS, TOS, and OSI values between grade 1 and 2 embryos, whereas pregnancy rates were significantly higher in patients who received grade 1 embryo transfer (P < 0.05). However, the follicular fluid TAS levels were significantly lower in smoking patients than in those who did not smoke; TOS and OSI levels were significantly higher.
CONCLUSIONS: This study showed that exposure to oxidative stress might be a causative factor for infertility. In addition, ROS decreased the level of TAS by increasing OSI in the follicular fluid; thus, antioxidant supplementation might be a necessity.