■幽门螺杆菌引起的持续性感染(H.pylori),对抗生素治疗有抗药性,日益引起全球公共卫生关注。已知生物膜形成与持续性感染有关,因为其在增强抗微生物剂抗性和许多病原菌的耐受性中的作用。
■本研究旨在评估幽门螺杆菌临床分离株的生物膜形成及其对抗生素根除的影响。
■厚度,形态学,使用共聚焦激光扫描显微镜检查了来自9个幽门螺杆菌菌株的生物膜的结构,扫描电子显微镜,和透射电子显微镜。随后,通过测定阿莫西林的最小抑制浓度和最小生物膜根除浓度来评估浮游细菌和生物膜细菌的敏感性,克拉霉素,左氧氟沙星,还有四环素.
■结果显示菌株之间的生物膜厚度和密度不同,其特征是存在许多缠绕和连接细菌细胞的细丝。此外,几例表现出基于MIC测量的磁化率,但根据MBEC测量的电阻,MBEC表明较高的耐药率。Pearson相关分析显示生物膜厚度与MBEC结果呈正相关(0■幽门螺杆菌的不同菌株表现出它们释放外膜囊泡(OMV)和形成生物膜的能力的差异。生物膜形成可以影响阿莫西林和四环素在根除易感细菌菌株中的有效性。
UNASSIGNED: Persistent infections caused by Helicobacter pylori (H. pylori), which are resistant to antibiotic treatment, pose a growing global public health concern. Biofilm formation is known to be associated with persistent infections due to its role in enhancing antimicrobial resistance and the tolerance of many pathogenic bacteria.
UNASSIGNED: This study aims to evaluate the biofilm formation of clinical isolates of H. pylori and its impact on antibiotic eradication.
UNASSIGNED: The thickness, morphology, and structure of biofilms derived from nine H. pylori strains were examined using confocal laser scanning microscopy, scanning electron microscopy, and transmission electron microscopy. Subsequently, the susceptibility of both planktonic and biofilm bacteria was assessed through the determination of minimum inhibitory concentration and minimum biofilm eradication concentration for amoxicillin, clarithromycin, levofloxacin, and tetracycline.
UNASSIGNED: The results revealed varying biofilm thicknesses and densities among the strains, characterised by the presence of numerous filaments intertwining and connecting bacterial cells. Additionally, several cases exhibited susceptibility based on MIC measurements but resistance according to MBEC measurements, with MBEC indicating a higher resistance rate. Pearson Correlation analysis demonstrated a positive correlation between biofilm thickness and MBEC results (0 < r < 1), notably significant for amoxicillin (r = 0.801, P = 0.009) and tetracycline (r = 0.696, P = 0.037).
UNASSIGNED: Different strains of H. pylori exhibit variations in their capacity to release outer membrane vesicles (OMVs) and form biofilms. Biofilm formation can influence the effectiveness of amoxicillin and tetracycline in eradicating susceptible bacterial strains.