Abstract：
OBJECTIVE: Urinary tract infections are the most common hospital-acquired infection, 80% are associated with catheterisation. Diagnostic methods may influence the reported identities of these pathogens, and phenotypic testing under laboratory conditions may not reflect infection phenotypes. This study aimed to evaluate the efficacy of diagnostic methods and whether medium composition alters phenotypes by characterizing catheter-associated urinary tract infection isolates from a UK hospital.
RESULTS: We compared five bacterial identification methods, including biochemical testing, MALDI biotyping, and genome sequencing, finding differences in genus or species level identifications. Antibiotic susceptibility comparisons between phenotypic assays and genomic predictions showed high agreement only in multidrug-resistant strains. To determine whether growth rate and biofilm formation were affected by medium composition, strains were grown in both planktonic and biofilm states. Low planktonic growth and significant biofilm formation were observed in artificial urine compared to rich laboratory media, underscoring the importance of assay design.
CONCLUSIONS: This study highlights the risks of relying on a single diagnostic method for species identification, advocating for whole-genome sequencing for accuracy. It emphasizes the continued importance of phenotypic methods in understanding antibiotic resistance in clinical settings and the need for characterization conditions that mirror those encountered by pathogens in the body.
RESULTS: We compared five bacterial identification methods, including biochemical testing, MALDI biotyping, and genome sequencing, finding differences in genus or species level identifications. Antibiotic susceptibility comparisons between phenotypic assays and genomic predictions showed high agreement only in multidrug-resistant strains. To determine whether growth rate and biofilm formation were affected by medium composition, strains were grown in both planktonic and biofilm states. Low planktonic growth and significant biofilm formation were observed in artificial urine compared to rich laboratory media, underscoring the importance of assay design.
CONCLUSIONS: This study highlights the risks of relying on a single diagnostic method for species identification, advocating for whole-genome sequencing for accuracy. It emphasizes the continued importance of phenotypic methods in understanding antibiotic resistance in clinical settings and the need for characterization conditions that mirror those encountered by pathogens in the body.
摘要：
目的：尿路感染是最常见的医院获得性感染，80%与导管插入有关。诊断方法可能会影响这些病原体的报告身份，实验室条件下的表型检测可能无法反映感染表型。这项研究旨在通过表征英国医院的导管相关尿路感染分离株，评估诊断方法的有效性以及培养基成分是否改变表型。
结果：我们比较了五种细菌鉴定方法，包括生化检测,MALDI生物分型，和基因组测序，发现属或种水平识别的差异。表型测定和基因组预测之间的抗生素敏感性比较仅在多药耐药菌株中显示高度一致。为了确定生长速率和生物膜形成是否受培养基组成的影响，菌株在浮游和生物膜状态下生长。与丰富的实验室培养基相比，在人工尿液中观察到低浮游生长和显着的生物膜形成，强调分析设计的重要性。
结论：这项研究强调了依赖单一诊断方法进行物种鉴定的风险，提倡全基因组测序的准确性。它强调了表型方法在了解临床环境中的抗生素耐药性方面的持续重要性，以及对反映体内病原体遇到的表征条件的需求。
结果：我们比较了五种细菌鉴定方法，包括生化检测,MALDI生物分型，和基因组测序，发现属或种水平识别的差异。表型测定和基因组预测之间的抗生素敏感性比较仅在多药耐药菌株中显示高度一致。为了确定生长速率和生物膜形成是否受培养基组成的影响，菌株在浮游和生物膜状态下生长。与丰富的实验室培养基相比，在人工尿液中观察到低浮游生长和显着的生物膜形成，强调分析设计的重要性。
结论：这项研究强调了依赖单一诊断方法进行物种鉴定的风险，提倡全基因组测序的准确性。它强调了表型方法在了解临床环境中的抗生素耐药性方面的持续重要性，以及对反映体内病原体遇到的表征条件的需求。
