PDF(Pubmed)
Abstract：
In response to the global rise in antibiotic resistance and the prevalence of bacterial biofilm-related infections, the antibacterial efficacy of methanolic, ethanolic, and aqueous extracts of 18 Lamiaceae plants from Serbia was evaluated. The total coumarins and triterpenes were detected spectrophotometrically, while a microdilution assay measured their effects on bacterial growth. Additionally, the impact of these extracts was assessed on Pseudomonas aeruginosa PAO1 adhesion and invasion in human fibroblasts and biofilm formation and degradation. The alcoholic extracts had the highest phytochemical content, with Teucrium montanum and Lavandula angustifolia being the richest in coumarins and triterpenes, respectively. Gram-positive bacteria, particularly Bacillus subtilis, were more susceptible to the extracts. Hyssopus officinalis ethanolic and Sideritis scardica methanolic extracts inhibited bacterial growth the most efficiently. Although the extracts did not inhibit bacterial adhesion, most ethanolic extracts significantly reduced bacterial invasion. Origanum vulgare and H. officinalis ethanolic extracts significantly inhibited biofilm formation, while Teucrium chamaedrys extract was the most active in biofilm degradation. This study significantly contributes to the literature by examining the antibacterial activity of Lamiaceae extracts, addressing major literature gaps, and underscoring their antibacterial potential, particularly Satureja montana and O. vulgare ethanolic extracts, linking their efficacy to coumarins and triterpenes.
摘要：
为了应对全球抗生素耐药性的上升和细菌生物膜相关感染的流行，甲醇的抗菌功效，乙醇，并对来自塞尔维亚的18种唇形科植物的水提取物进行了评估。用分光光度法检测总香豆素和三萜，而微量稀释试验测量了它们对细菌生长的影响。此外,评估了这些提取物对铜绿假单胞菌PAO1在人成纤维细胞中的粘附和侵袭以及生物膜形成和降解的影响。酒精提取物的植物化学物质含量最高，香豆素和三萜中香豆素和三萜含量最丰富，分别。革兰氏阳性菌,特别是枯草芽孢杆菌,对提取物更敏感。Hyssopusofficinalis乙醇和Sideritissardica甲醇提取物最有效地抑制细菌生长。虽然提取物没有抑制细菌粘附，大多数乙醇提取物显着减少细菌入侵。Ohiganumvulgare和H.officinalis乙醇提取物显着抑制生物膜的形成，而Teucriumchamaedrys提取物在生物膜降解中最活跃。这项研究通过检查唇形科提取物的抗菌活性显着有助于文献，解决主要的文献空白，强调它们的抗菌潜力，特别是Saturejamontana和O.vulgare乙醇提取物，将它们的功效与香豆素和三萜联系起来。
