在获得持久性循环病毒如polerovirus后,病毒颗粒通过血淋巴通过中肠和唾液腺的蚜虫膜屏障进行细胞转运。这种复杂的相互作用可以影响蚜虫的行为和适应性,并诱导相关的基因表达。可通过组学方法如转录组学评估差异基因表达。以前进行的蚜虫转录组研究仅使用一种宿主物种作为病毒接种物的来源。病毒通常具有备用宿主。因此,目前尚不清楚感染同一病毒分离株的替代宿主如何改变病毒载体中的基因表达。为了解决这个问题,本研究对从不同宿主物种获得病毒的含病毒蚜虫进行了转录组分析。一种polerovirus,棉花卷叶矮病毒(CLRDV),在棉花蚜虫中诱导基因表达,AphisgossypiiGlover,使用四个备用主机进行评估,viz.,棉花,芙蓉,秋葵,还有带刺的Sida.在总共2,942个差异表达基因(DEGs)中,在从受感染的棉花获得CLRDV的A.gossypii中鉴定出750、310、1、193和689个基因,芙蓉,秋葵,和多刺的西达,分别,与在未感染宿主上发育的非有毒蚜虫相比。从棉花获得CLRDV后,蚜虫基因的过表达比例高于低表达比例,芙蓉,还有带刺的Sida.相比之下,从秋葵植物获得CLRDV后,低表达的蚜虫基因多于过表达的基因。只有四种常见的DEG(热休克蛋白,幼年激素酸性O-甲基转移酶,和两个未注释的基因)在来自四个备用宿主的含病毒蚜虫中鉴定。基因本体论(GO)富集剖析和京都基因和基因组百科全书(KEGG)注解,CLRDV引诱的DEGs在蚜虫中的获得与病毒感染有关,信号转导,免疫系统,和健身。然而,这些诱导的变化在4个备用宿主中并不一致.这些数据表明,尽管感染了相同的病毒分离株,但替代宿主可能会差异影响蚜虫的基因表达,并可能影响蚜虫的行为和适应性。
Upon acquisition of persistent circulative viruses such as poleroviruses, the virus particles transcytose through membrane barriers of aphids at the midgut and salivary glands via hemolymph. Such intricate interactions can influence aphid behavior and fitness and induce associated gene expression in viruliferous aphids. Differential gene expression can be evaluated by omics approaches such as transcriptomics. Previously conducted aphid transcriptome studies used only one host species as the source of virus inoculum. Viruses typically have alternate hosts. Hence, it is not clear how alternate hosts infected with the same virus isolate alter gene expression in viruliferous vectors. To address the question, this study conducted a transcriptome analysis of viruliferous aphids that acquired the virus from different host species. A polerovirus, cotton leafroll dwarf virus (CLRDV), which induced gene expression in the cotton aphid, Aphis gossypii Glover, was assessed using four alternate hosts, viz., cotton, hibiscus, okra, and prickly sida. Among a total of 2,942 differentially expressed genes (DEGs), 750, 310, 1,193, and 689 genes were identified in A. gossypii that acquired CLRDV from infected cotton, hibiscus, okra, and prickly sida, respectively, compared with non-viruliferous aphids that developed on non-infected hosts. A higher proportion of aphid genes were overexpressed than underexpressed following CLRDV acquisition from cotton, hibiscus, and prickly sida. In contrast, more aphid genes were underexpressed than overexpressed following CLRDV acquisition from okra plants. Only four common DEGs (heat shock protein, juvenile hormone acid O-methyltransferase, and two unannotated genes) were identified among viruliferous aphids from four alternate hosts. Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations indicated that the acquisition of CLRDV induced DEGs in aphids associated with virus infection, signal transduction, immune systems, and fitness. However, these induced changes were not consistent across four alternate hosts. These data indicate that alternate hosts could differentially influence gene expression in aphids and presumably aphid behavior and fitness despite being infected with the same virus isolate.