Aphisgossypii是一种世界性的农业害虫,对多种农药产生了抗性。Dimprodydapaz是一种新的脊索器官调节剂,已注册用于控制包括A.gossypii在内的吸汁昆虫。为了有效地将Dripropyridaz应用于A.gossypii控制,有必要阐明Dempropyridaz对棉蚜虫的毒性作用。在本研究中,Dripropyridaz对摄食行为的影响,研究了棉草的运动能力和生物学参数。生物测定结果表明,Dempropyridaz对棉草具有良好的杀虫活性,暴露后72小时LC50为1.91mg/L。此外,Dinpropyridaz治疗的A.gossypii表现出明显的脱水和枯萎中毒症状。通过轻触实验和喂食实验,发现DiPropyridaz治疗对A.gossypii的运动能力和摄食行为有重大不利影响。与对照组相比,被治疗的棉草的协调运动能力减弱,此外,棉草的摄食行为受到抑制。摄食率下降62.00%,用50.33mg/Ldimpropyridaz治疗24h后分别为64.00%和71.67%,48h和72h,分别。尤其是,EPG记录显示,细胞内探针穿刺次数和韧皮部汁液摄入和并发流涎的总持续时间大幅减少,而暴露于dimpropyridaz后,非探查的总持续时间增加。此外,Dinpropyridaz的LC10和LC30处理显着降低了F0的寿命和繁殖力,并导致F0的相对适合度分别降低至0.48和0.32。F1代的净繁殖率(R0)和平均世代时间(T)也显著降低,此外,繁殖时间显著缩短。此外,在用LC30DiPropyridaz治疗后72小时,棉花蚜虫JHEH和USP基因表达水平显著提高,而FOXO的表达,INR,EcR和INRS下降。这些结果为阐明Dempropyridaz对棉蚜虫的毒理学提供了依据。也有利于使用DiPropyridaz有效防治棉蚜虫。
Aphis gossypii is a worldwide agricultural pest insect that has developed resistance to multiple pesticides. Dimpropyridaz is a new chordotonal organ regulator and has been registered for control of sap-sucking insects including A. gossypii. For the aim to effectively apply dimpropyridaz for A. gossypii control, it is necessary to clarify the toxic effects of dimpropyridaz on cotton aphids. In the present study, the effects of dimpropyridaz on feeding behavior, locomotivity and biological parameters of A. gossypii were investigated. The bioassay results showed that dimpropyridaz had good insecticidal activity against A. gossypii, with LC50 as 1.91 mg/L at 72 h post exposure. Moreover, the dimpropyridaz treated A. gossypii showed obvious poisoning symptoms of dehydration and shrivel. Through the gentle-touch experiment and feeding experiment, it was found that dimpropyridaz treatment had significant adverse impacts on the locomotivity and feeding behavior of A. gossypii. Compared with the control group, the coordinated movement ability of the treated A. gossypii attenuated, moreover the feeding behavior of A. gossypii was inhibited. The feeding rate decreased by 62.00%, 64.00% and 71.67% after treatment with 50.33 mg/L dimpropyridaz for 24 h, 48 h and 72 h, respectively. Especially, EPG recordings showed that the number of intracellular stylet puncture and the total duration of phloem sap ingestion and concurrent salivation decreased substantially, while the total duration of non-probing increased after exposure to dimpropyridaz. Furthermore, the treatments with LC10 and LC30 of dimpropyridaz significantly reduced the longevity and fecundity of F0, and led to a decrease of the relative fitness of F0 to 0.48 and 0.32, respectively. The net reproductive rate (R0) and mean generation time (T) of F1 generation were also significantly reduced, moreover the duration of reproduction was significantly shortened. In addition, at 72 h post treatment with LC30 dimpropyridaz, the gene expression levels of JHEH and USP of cotton aphids significantly increased, while the expression of FOXO, INR, EcR and INRS decreased. These results provide basis for clarifying the toxicology of dimpropyridaz to cotton aphids, and also are beneficial for effective control of cotton aphid using dimpropyridaz.