Abstract：
Aphis gossypii, a globally distributed and economically significant pest of several crops, is known to infest a wide range of host plants. Heat shock proteins (Hsps), acting as molecular chaperones, are essential for the insect\'s environmental stress responses. The present study investigated the molecular characteristics and expression patterns of AgHsp70, a heat shock protein gene, in Aphis gossypii. Our phylogenetic analysis revealed that AgHsp70 shared high similarity with homologs from other insects, suggesting a conserved function across species. The developmental expression profiles of AgHsp70 in A. gossypii showed that the highest transcript levels were observed in the fourth instar nymphs, while the lowest levels were detected in the third instar nymphs. Heat stress and exposure to four different xenobiotics (2-tridecanone, tannic acid, gossypol, and flupyradifurone (4-[(2,2-difluoroethyl)amino]-2(5H)-furanone)) significantly up-regulated AgHsp70 expression. Knockdown of AgHsp70 using RNAi obviously increased the susceptibility of cotton aphids to 2-tridecanone, gossypol and flupyradifurone. Dual-luciferase reporter assays revealed that gossypol and flupyradifurone significantly enhanced the promoter activity of AgHsp70 at a concentration of 10 mg/L. Furthermore, we identified the transcription factor heat shock factor (HSF) as a regulator of AgHsp70, as silencing AgHSF reduced AgHsp70 expression. Our results shed light on the role of AgHsp70 in xenobiotic adaptation and thermo-tolerance.
摘要：
山雀,一种全球分布且经济意义重大的几种作物害虫，已知会感染多种寄主植物。热休克蛋白（Hsps），作为分子伴侣，对昆虫的环境应激反应至关重要。本研究调查了热休克蛋白基因AgHsp70的分子特征和表达模式，在Aphisgossypii.我们的系统发育分析表明，AgHsp70与其他昆虫的同源物具有很高的相似性，表明跨物种的保守功能。AgHsp70在棉草中的发育表达谱显示，在第四龄若虫中观察到最高的转录水平，而在第三龄若虫中检测到最低水平。热应激和暴露于四种不同的外源性物质(2-十三烷酮，鞣酸,棉酚,和氟吡喃酮(4-[(2,2-二氟乙基)氨基]-2(5H)-呋喃酮)显著上调AgHsp70表达。用RNAi敲除AgHsp70能明显增加棉蚜虫对2-十三烷酮的敏感性,棉酚和氟吡喃酮。双荧光素酶报告基因实验表明，棉酚和氟吡唑酮在10mg/L的浓度下显着增强了AgHsp70的启动子活性。此外，我们将转录因子热休克因子(HSF)鉴定为AgHsp70的调节因子,因为沉默AgHSF会降低AgHsp70的表达.我们的结果揭示了AgHsp70在异种生物适应和耐热性中的作用。
