PDF(Pubmed)
Abstract：
Negeviruses are insect-specific enveloped RNA viruses that exhibit a wide geographic distribution. A novel nege-like virus, tentatively named Aphis gossypii nege-like virus (AGNLV, GenBank: OR880429.1), was isolated from aphids (Aphis gossypii) in Lijiang City, Yunnan, China. AGNLV has a genome sequence of 9258 nt (excluding the polyA tail) encoding three open reading frames (ORFs). ORF1 (7149 nt) encodes a viral methyltransferase, a viral RNA helicase, and an RNA-dependent RNA polymerase. ORF2 (1422 nt) encodes a DiSB-ORF2_chro domain and ORF3 encodes an SP24 domain. The genome sequence of AGNLV shares the highest nucleotide identity of 60.0% and 59.5% with Wuhan house centipede virus 1 (WHCV1) and Astegopteryx formosana nege-like virus (AFNLV), respectively. Phylogenetic analysis based on the RNA-dependent RNA polymerase shows that AGNLV is clustered with other negeviruses and nege-like viruses discovered in aphids, forming a distinct \"unclassified clade\". Interestingly, AGNLV only encodes three ORFs, whereas AFNLV and WHCV1 have four ORFs. Structure and transmembrane domain predictions show the presence of eight alpha helices and five transmembrane helices in the AGNLV ORF3. Translational enhancement of the AGNLV 5\' UTR was similar to that of the 5\' UTR of plant viruses. Our findings provide evidence of the diversity and structure of nege-like viruses and are the first record of such a virus from a member of the genus Aphis.
摘要：
网状病毒是表现出广泛地理分布的昆虫特异性包膜RNA病毒。一种新型的类神经病毒,暂定名为阿菲斯戈西皮尼格样病毒(AGNLV，GenBank:OR880429.1),从丽江市的蚜虫（Aphisgossypii）中分离出来，云南,中国。AGNLV具有编码三个开放阅读框(ORF)的9258nt(不包括polyA尾)的基因组序列。ORF1（7149nt）编码病毒甲基转移酶，病毒RNA解旋酶,和RNA依赖性RNA聚合酶。ORF2(1422nt)编码DiSB-ORF2_chro结构域,ORF3编码SP24结构域。AGNLV的基因组序列与武汉家cent病毒1（WHCV1）和Astegopteryxformosananege样病毒（AFNLV）的核苷酸同一性最高，分别为60.0％和59.5％，分别。基于RNA依赖性RNA聚合酶的系统发育分析表明，AGNLV与蚜虫中发现的其他negesirus和nege样病毒成簇，形成一个独特的“未分类进化枝”。有趣的是,AGNLV只编码三个ORF,而AFNLV和WHCV1有四个ORF。结构和跨膜结构域预测显示在AGNLVORF3中存在八个α螺旋和五个跨膜螺旋。AGNLV5'UTR的翻译增强与植物病毒的5'UTR相似。我们的发现提供了类蛇病毒的多样性和结构的证据，并且是Aphis属成员中此类病毒的第一个记录。
