由于其优异的生物相容性和易于生物降解,水母明胶作为水凝胶已经受到关注。然而,由水母明胶生产的水凝胶尚未被充分表征。因此,这项研究旨在生产一种基于明胶的水母水凝胶。脱盐水母副产品产生的明胶随样品的部分和提取时间而变化。表征了明胶:戊二醛比例为10:0.25、10:0.50和10:1.00(v/v)的水凝胶,并测定其头孢唑啉的释放能力。明胶提取的最佳条件和用于开发水母水凝胶(JGel)的选择包括使用脱盐水母副产品的伞形部分提取24小时(WU24),这产生了最高的凝胶强度(460.02克),粘度(24.45cP),胶凝温度(12.70°C),和熔化温度(22.48°C)。胶原蛋白α-1(XXVIII)链A的数量,胶原蛋白α-1(XXI)链,WU24中的胶原蛋白α-2(IX)链可能会影响其凝胶特性。增加JGel中的戊二醛含量通过减少蛋白质链之间的空间和凝胶溶胀来增加凝胶分数,因为戊二醛与侧向氨基酸残基结合并产生更强的网络。在8小时,JGel(10:0.25)中80%以上的头孢唑啉被释放,其释放量高于牛水凝胶(52.81%)和鱼水凝胶(54.04%)。这项研究是第一份专注于使用戊二醛作为交联剂生产JGel的报告。
Due to its excellent biocompatibility and ease of biodegradation, jellyfish gelatin has gained attention as a hydrogel. However, hydrogel produced from jellyfish gelatin has not yet been sufficiently characterized. Therefore, this research aims to produce a jellyfish gelatin-based hydrogel. The gelatin produced from desalted jellyfish by-products varied with the part of the specimen and extraction time. Hydrogels with gelatin:
glutaraldehyde ratios of 10:0.25, 10:0.50, and 10:1.00 (v/v) were characterized, and their cefazolin release ability was determined. The optimal conditions for gelatin extraction and chosen for the development of jellyfish hydrogels (JGel) included the use of the umbrella part of desalted jellyfish by-products extracted for 24 h (WU24), which yielded the highest gel strength (460.02 g), viscosity (24.45 cP), gelling temperature (12.70 °C), and melting temperature (22.48 °C). The quantities of collagen alpha-1(XXVIII) chain A, collagen alpha-1(XXI) chain, and collagen alpha-2(IX) chain in WU24 may influence its gel properties. Increasing the
glutaraldehyde content in JGel increased the gel fraction by decreasing the space between the protein chains and gel swelling, as
glutaraldehyde binds with lateral amino acid residues and produces a stronger network. At 8 h, more than 80% of the cefazolin in JGel (10:0.25) was released, which was higher than that released from bovine hydrogel (52.81%) and fish hydrogel (54.04%). This research is the first report focused on the production of JGel using
glutaraldehyde as a cross-linking agent.