Abstract:
The aim of this study was to test the inactivation of viruses on germ carriers of different types of wood using a disinfectant in order to assess the biosafety of wood as a building material in animal husbandry. The laboratory disinfectant efficacy tests were based on German testing guidelines and current European standards. Five different types of wood germ carriers, i.e., spruce (Picea abies), pine (Pinus sylvestris), poplar (Populus sp.), beech (Fagus sylvatica) and Douglas fir (Pseudotsuga menziesii), were inoculated with enveloped or non-enveloped viruses and then treated with one of three different disinfectants. The results revealed that intact, fine-sawn timber with a low roughness depth can be effectively inactivated. Peracetic acid proved to be the most effective disinfectant across all tests. Regardless of the pathogen and the type of wood, a concentration of 0.1% of the pure substance at a temperature of 10 °C and an exposure time of one hour can be recommended. At a temperature of -10 °C, a concentration of 0.75% is recommended. The basic chemicals formic acid and glutaraldehyde demonstrated only limited effectiveness overall. The synergistic effects of various wood components on the inactivation of viruses offer potential for further investigation. Disinfectant tests should also be conclusively verified in field trials to ensure that the results from standardised laboratory tests can be transferred to real stable conditions.
摘要:
这项研究的目的是使用消毒剂测试不同类型木材的细菌携带者对病毒的灭活,以评估木材作为畜牧业建筑材料的生物安全性。实验室消毒剂功效测试基于德国测试指南和当前的欧洲标准。五种不同类型的木胚芽载体,即,云杉(云杉),松树(樟子松),杨树(杨树。),山毛榉(Fagussylvatica)和道格拉斯冷杉(Pseudotsugamenziesii),用包膜或无包膜病毒接种,然后用三种不同的消毒剂之一处理。结果显示,完好无损,具有低粗糙度深度的细锯木材可以有效地灭活。在所有测试中,过氧乙酸被证明是最有效的消毒剂。不管病原体和木材的类型,在10°C的温度和1小时的暴露时间下,可以推荐纯物质的浓度为0.1%。在-10°C的温度下,建议浓度为0.75%。碱性化学物质甲酸和戊二醛总体上仅表现出有限的有效性。各种木材成分对病毒灭活的协同作用为进一步研究提供了潜力。消毒剂测试也应在现场试验中进行最终验证,以确保标准化实验室测试的结果可以转移到真正稳定的条件。