冬虫夏草是蚂蚁常见的致病真菌。一个新物种,O.Fusiformispora,基于来自五个基因的形态学和系统发育证据进行了描述(SSU,LSU,TEF1α,RPB1和RPB2)。梭形孢子的整个基因组,O.重叠群,O.Subtiliphialida,O.Satoi,O.flabellata,O.acrosasca,和O.camponoti-leonardi进行了测序和注释,并与O.landeralissensulato中其他物种的全基因组序列进行了比较。12个物种的基本全基因组特征表明,相关物种具有相似的GC含量和基因组大小。AntiSMASH和局部BLAST分析显示,推定的SMBGC的数量和类型,NPPS,PKS,12个物种的杂种PKS-NRPS结构域在同一属的不同物种之间存在显着差异。五个化合物的推定BGC,即,NG-391,lucilactaene,希格西宁B,吡啶甲酸A,并挖出了吡喃苷E。NG-391和lucilactaene是fusarinC的7-去甲基类似物。这12个基因组有共同的结构域,如KS-AT-DH-MT-ER-KR-ACP和SAT-KS-AT-PT-ACP-ACP-Te。SAT-KS-AT-PT-ACP-ACP-Te的ML和BI树与12种的多基因系统发育树高度一致。本研究提供了一种获得O.leneralissensulato物种及其在活文化基础上形成的无性的活文化的方法,这对于将来进一步研究O.landenalissensulato物种具有重要价值,同时也为进一步分析奥氏次生代谢产物奠定了基础。
Ophiocordyceps unilateralis sensu lato is a common pathogenic fungus of ants. A new species, O. fusiformispora, was described based on morphology and phylogenetic evidence from five genes (SSU, LSU, TEF1α, RPB1, and RPB2). The whole genomes of O. fusiformispora, O. contiispora, O. subtiliphialida, O. satoi, O. flabellata, O. acroasca, and O. camponoti-leonardi were sequenced and annotated and compared with whole genome sequences of other species in O. unilateralis sensu lato. The basic genome-wide characteristics of the 12 species showed that the related species had similar GC content and genome size. AntiSMASH and local BLAST analyses revealed that the number and types of putative SM BGCs, NPPS, PKS, and hybrid PKS-NRPS domains for the 12 species differed significantly among different species in the same genus. The putative BGC of five compounds, namely, NG-391, lucilactaene, higginsianin B, pyripyropene A, and pyranonigrin E were excavated. NG-391 and lucilactaene were 7-desmethyl analogs of fusarin C. Furthermore, the 12 genomes had common domains, such as KS-AT-DH-MT-ER-KR-ACP and SAT-KS-AT-PT-ACP-ACP-Te. The ML and BI trees of SAT-KS-AT-PT-ACP-ACP-Te were highly consistent with the multigene phylogenetic tree in the 12 species. This study provided a method to obtain the living culture of O. unilateralis sensu lato species and its asexual formed on the basis of living culture, which was of great value for further study of O. unilateralis sensu lato species in the future, and also laid a foundation for further analysis of secondary metabolites of O. unilateralis sensu lato.